Inhibition of macrophage invasion by monoclonal antibodies specific to Leishmania (Viannia) braziliensis promastigotes and characterisation of their antigens

Inhibition of macrophage invasion by monoclonal antibodies specific to Leishmania (Viannia) braziliensis promastigotes and characterisation of their antigens

Author Silveira, TGV Google Scholar
Suzuki, E. Google Scholar
Takahashi, H. K. Google Scholar
Straus, A. H. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Monoclonal antibodies that specifically recognise Leishmania (Viannia) braziliensis promastigotes were produced and termed SST-2, SST-3 and SST-4. SST-2 recognises a conformational epitope present in a 24-28 kDa doublet and in a 72 kDa component as verified by Western blotting. Indirect immunofluorescence showed that the antigen recognised by SST-2 is distributed homogeneously on the parasite surface. SST-3 recognises a flagellar glycoprotein of similar to 180 kDa. the reactivity of this mAb was abolished by sodium in-periodate treatment. indicating that SST-3 reacts with a carbohydrate epitope of the 180 kDa antigen. SST-4 recognises a conformational epitope of a 98 kDa antigen. SST-2, SST-3 and SST-4 were specific to L. (V.) braziliensis promastigote forms. Indirect immunofluorescence did not show reactivity of SST-2 or SST-3 with amastigotes of L, (V) braziliensis, or with promastigotes of Leishmania (Viannia) panamensis, Leishmania (Viannia) guyanensis, Leishmania (Viannia) naiffi, Leishmania (Viannia) lainsoni, Leishmania (Leishmania) amazonensis, Leishmania (Leishmania) major. or Leishmania (Leishmania) chagasi. We also evaluated the involvement of SST-2, SST-3 and SST-4 antigens in parasite-macrophage interaction. Fab fragments of SST-3 and SST-4 significantly inhibited the infectivity of L. (V.) braziliensis promastigotes to mouse peritoneal macrophages. (C) 2001 Australian Society for Parasitology Inc. Published by Elsevier B.V. All rights reserved.
Keywords Leishmania (Viannia) braziliensis
monoclonal antibody
flagella
Leishmania
infectivity
Language English
Date 2001-11-01
Published in International Journal for Parasitology. Oxford: Elsevier B.V., v. 31, n. 13, p. 1451-1458, 2001.
ISSN 0020-7519 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 1451-1458
Origin http://dx.doi.org/10.1016/S0020-7519(01)00269-7
Access rights Closed access
Type Article
Web of Science ID WOS:000171912800007
URI http://repositorio.unifesp.br/handle/11600/26656

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