The contribution of 700,000 ORF sequence tags to the definition of the human transcriptome

The contribution of 700,000 ORF sequence tags to the definition of the human transcriptome

Autor Camargo, A. A. Google Scholar
Samaia, HPB Google Scholar
Dias-Neto, E. Google Scholar
Simao, D. F. Google Scholar
Migotto, I. A. Google Scholar
Briones, Marcelo Ribeiro da Silva Autor UNIFESP Google Scholar
Costa, F. F. Google Scholar
Nagai, M. A. Google Scholar
Verjovski-Almeida, S. Google Scholar
Zago, M. A. Google Scholar
Andrade, Luiz Eduardo Coelho Autor UNIFESP Google Scholar
Carrer, H. Google Scholar
El-Dorry, HFA Google Scholar
Espreafico, E. M. Google Scholar
Habr-Gama, A. Google Scholar
Giannella-Neto, D. Google Scholar
Goldman, G. H. Google Scholar
Gruber, A. Google Scholar
Hackel, C. Google Scholar
Kimura, Edna Teruko Autor UNIFESP Google Scholar
Maciel, Rui Monteiro de Barros Autor UNIFESP Google Scholar
Marie, SKN Google Scholar
Martins, EAL Google Scholar
Nobrega, M. P. Google Scholar
Paco-Larson, M. L. Google Scholar
Pardini, MIMC Google Scholar
Pereira, G. G. Google Scholar
Pesquero, João Bosco Autor UNIFESP Google Scholar
Rodrigues, V Google Scholar
Rogatto, SR Google Scholar
Silva, Ismael Dale Cotrim Guerreiro da Autor UNIFESP Google Scholar
Sogayar, M. C. Google Scholar
Sonati, MDF Google Scholar
Tajara, E. H. Google Scholar
Valentini, Sandro Roberto Google Scholar
Alberto, F. L. Google Scholar
Amaral, MEJ Google Scholar
Aneas, I Google Scholar
Arnaldi, Liliane Aparecida Teixeira Autor UNIFESP Google Scholar
Assis, A. M. de Google Scholar
Bengtson, M. H. Google Scholar
Bergamo, N. A. Google Scholar
Bombonato, V Google Scholar
Camargo, MER de Google Scholar
Canevari, R. A. Google Scholar
Carraro, D. M. Google Scholar
Cerutti, Janete Maria Autor UNIFESP Google Scholar
Correa, MLC Google Scholar
Correa, RFR Google Scholar
Costa, MCR Google Scholar
Curcio, C. Google Scholar
Hokama, POM Google Scholar
Ferreira, AJS Google Scholar
Furuzawa, Gilberto Koiti Autor UNIFESP Google Scholar
Gushiken, T. Google Scholar
Ho, P. L. Google Scholar
Kimura, E. Google Scholar
Krieger, J. E. Google Scholar
Leite, LCC Google Scholar
Majumder, P. Google Scholar
Marins, M. Google Scholar
Marques, E. R. Google Scholar
Melo, Analy Salles de Azevedo Autor UNIFESP Google Scholar
Melo, M. Google Scholar
Mestriner, Carlos Alberto Google Scholar
Miracca, E. C. Google Scholar
Miranda, D. C. Google Scholar
Nascimento, Ana Lucia Tabet Oller do Google Scholar
Nobrega, F. G. Google Scholar
Ojopi, EPB Google Scholar
Pandolfi, JRC Google Scholar
Pessoa, Luciana Gilbert Autor UNIFESP Google Scholar
Prevedel, A. C. Google Scholar
Rahal, P. Google Scholar
Rainho, C. A. Google Scholar
Reis, EMR Google Scholar
Ribeiro, M. L. Google Scholar
Ros, N. da Google Scholar
Sa, R. G. de Google Scholar
Sales, M. M. Google Scholar
Sant'anna, S. C. Google Scholar
Santos, M. L. dos Google Scholar
Silva, A. M. da Google Scholar
Silva, Neusa Pereira da Autor UNIFESP Google Scholar
Silva, W. A. Google Scholar
Silveira, R. A. da Google Scholar
Sousa, J. F. Google Scholar
Stecconi, D. Google Scholar
Tsukumo, F. Google Scholar
Valente, V Google Scholar
Soares, F. Google Scholar
Moreira, E. S. Google Scholar
Nunes, D. N. Google Scholar
Correa, R. G. Google Scholar
Zalcberg, H. Google Scholar
Carvalho, A. F. Google Scholar
Reis, LFL Google Scholar
Brentani, Ricardo Renzo Autor UNIFESP Google Scholar
Simpson, AJG Google Scholar
Souza, S. J. de Google Scholar
Instituição Ludwig Inst Canc Res
Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Campinas (UNICAMP)
Universidade de São Paulo (USP)
Fac Med Ribeirao Preto
Inst Butantan
Univ Vale Paraiba
Univ Estadual Paulista
Hosp AC Camargo Fund Antonio Prudente
Resumo open reading frame expressed sequences tags (ORESTES) differ from conventional ESTs by providing sequence data from the central protein coding portion of transcripts. We generated a total of 696,745 ORESTES sequences from 24 human tissues and used a subset of the data that correspond to a set of 15,095 full-length mRNAs as a means of assessing the efficiency of the strategy and its potential contribution to the definition of the human transcriptome. We estimate that ORESTES sampled over 80% of all highly and moderately expressed, and between 40% and 50% of rarely expressed, human genes. in our most thoroughly sequenced tissue, the breast, the 130,000 ORESTES generated are derived from transcripts from an estimated 70% of all genes expressed in that tissue, with an equally efficient representation of both highly and poorly expressed genes. in this respect, we find that the capacity of the ORESTES strategy both for gene discovery and shotgun transcript sequence generation significantly exceeds that of conventional ESTs. the distribution of ORESTES is such that many human transcripts are now represented by a scaffold of partial sequences distributed along the length of each gene product. the experimental joining of the scaffold components, by reverse transcription-PCR, represents a direct route to transcript finishing that may represent a useful alternative to full-length cDNA cloning.
Idioma Inglês
Data de publicação 2001-10-09
Publicado em Proceedings of the National Academy of Sciences of the United States of America. Washington: Natl Acad Sciences, v. 98, n. 21, p. 12103-12108, 2001.
ISSN 0027-8424 (Sherpa/Romeo, fator de impacto)
Publicador Natl Acad Sciences
Extensão 12103-12108
Fonte http://dx.doi.org/10.1073/pnas.201182798
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000171558900052
Endereço permanente http://repositorio.unifesp.br/handle/11600/26642

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