Comparative genomic hybridization analysis identifies gains of 1p35 similar to p36 and chromosome 19 in osteosarcoma

Comparative genomic hybridization analysis identifies gains of 1p35 similar to p36 and chromosome 19 in osteosarcoma

Autor Zielenska, M. Google Scholar
Bayani, Jane Google Scholar
Pandita, A. Google Scholar
Toledo, Silvia Regina Caminada de Autor UNIFESP Google Scholar
Marrano, P. Google Scholar
Andrade, Joyce Anderson Dufles Google Scholar
Petrilli, A. Google Scholar
Thorner, P. Google Scholar
Sorensen, P. Google Scholar
Squire, Jeremy A. Google Scholar
Instituição Univ Toronto
Ontario Canc Inst
Hosp Sick Children
Universidade Federal de São Paulo (UNIFESP)
British Columbia Childrens Hosp
Resumo Osteosarcomas (OS) are aggressive tumors of the bone and often have a poor prognosis, Conventional cytogenetic analyses of OS have revealed highly complex karyotypes, with numerous abnormalities. in this study, we analyzed 18 untreated OS tumors from 17 patients of the younger incidence age group by comparative genomic hybridization (CGH), 4 tumors by spectral karyotyping (SKY) and fluorescence in situ hybridization (FISH). Comparative genomic hybridization identified frequent copy number changes of the chromosomal region lp (10/17) and gain of part or all of chromosome 19(8/17). in addition gains were observed at 5p(3/17), Sq(3/17), 16p(3/17), and 17p(5/17) and losses at chromosomes 2q(3/17), 10(4/17) and 13(3/17). High level gains were detected in the 8q23-q24 region in two tumors as well as at 17p in one primary and a metastatic tumor. Minimal regions of gain were present at 1p35 similar to p36.3 (8/17): 5p14 similar to p15.2 (3/17), and 8q22 similar to q24.3 (3/17). SKY analysis demonstrated that OS has a complex pattern of clonal and non-clonal rearrangements and helped confirm the structural basis for the imbalances detected by CGH. Spectral karyotyping confirmed an overall pattern of chromosomal gain affecting 1p in all four tumors. Fluorescence in situ hybridization analysis from these tumors confirmed the gain of the 1p36 region in 2 tumors as determined by CGH analysis as well as the amplification of 8q. (C) 2001 Elsevier Science Inc. All rights reserved.
Idioma Inglês
Data 2001-10-01
Publicado em Cancer Genetics and Cytogenetics. New York: Elsevier B.V., v. 130, n. 1, p. 14-21, 2001.
ISSN 0165-4608 (Sherpa/Romeo, fator de impacto)
Editor Elsevier B.V.
Extensão 14-21
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000171742000003

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