Characterization of muscarinic acetylcholine receptors in the rat epididymis

Characterization of muscarinic acetylcholine receptors in the rat epididymis

Autor Marostica, E. Autor UNIFESP Google Scholar
Guaze, E. F. Autor UNIFESP Google Scholar
Avellar, MCW Autor UNIFESP Google Scholar
Porto, C. S. Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo The aim of the present study was to characterize the muscarinic acetylcholine receptor subtypes present in the caput and cauda of rat epididymis. the specific binding of [H-3]quinuclidinyl benzilate ([H-3]QNB) to epididymal membranes was time dependent, temperature dependent, and saturable. the cauda epididymis showed higher affinity to [H-3]QNB and higher muscarinic receptor density when compared to the caput region. the [H-3]QNB binding was tested in competition studies with different muscarinic receptor antagonists. Each antagonist tested displaced [H-3]QNB bound to caput and cauda epididymal membrane with similar affinity. Correlation among the negative logarithm of inhibition constant values (pK(i)) for these antagonists obtained in the epididymis with their correspondent published pK(i) values obtained in tissues that expressed each receptor subtype (M-1, M-2, M-3, and M-4) indicated that the muscarinic receptors present in caput and cauda epididymis belong to the muscarinic M, receptor subtype. When reverse transcription-polymerase chain reaction was used to identify muscarinic receptor mRNA subtypes in the epididymis, only m2 transcripts were detected in the caput region, while both m2 and m3 mRNA subtypes were observed in the cauda region. in conclusion, these results demonstrate that muscarinic receptors are present in the rat epididymis, with expression levels dependent on the region of the epididymis analyzed. Thus, the cholinergic neurotransmitter in the epididymis may be a factor controlling contractility and/or the luminal fluid microenvironment.
Palavra-chave catecholamines and acetylcholine
epididymis
male sexual function
Idioma Inglês
Data de publicação 2001-10-01
Publicado em Biology of Reproduction. Madison: Soc Study Reproduction, v. 65, n. 4, p. 1120-1126, 2001.
ISSN 0006-3363 (Sherpa/Romeo, fator de impacto)
Publicador Soc Study Reproduction
Extensão 1120-1126
Fonte http://dx.doi.org/10.1095/biolreprod65.4.1120
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000171320400018
Endereço permanente http://repositorio.unifesp.br/handle/11600/26631

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