Immunostimulatory DNA from Paracoccidioides brasiliensis acts as T-helper 1 promoter in susceptible mice

Immunostimulatory DNA from Paracoccidioides brasiliensis acts as T-helper 1 promoter in susceptible mice

Autor Souza, Marta Cristina Autor UNIFESP Google Scholar
Correa, Mariangela Autor UNIFESP Google Scholar
Almeida, Sandro Rogério de Autor UNIFESP Google Scholar
Lopes, Jose Daniel Autor UNIFESP Google Scholar
Camargo, Zoilo Pires de Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Th1 immune responses afford protection against some pathogens like the fungus R brasiliensis (P.b.), ethiological agent of Paracoccidioidomycosis (PCM). It is well known that nonmethylated CpG sequences from bacterial DNA have immunomodulatory properties and can be used as a Th1-promoting adjuvant. By analyzing the available gene sequences of P.b. we observed a high number of unmethylated CpG dinucleotides. in a murine model of the PCM infection, the isogenic mouse strain known to be susceptible presents a predominant Th2 pattern. in order to access the possibility of the genomic DNA to act as a Th1-promoting adjuvant, in vitro assays were made and indicated a significant increase in phagocytosis when the macrophages were stimulated with DNA from Rb. and in vivo assays of a decreased production of antibodies antigp43, the main antigen of the PCM system. the analysis of the antibody isotypes and the cytokine production suggested a Th1 modulation in the susceptible animals. Thus, when mice were infected with fungus plus synthetic oligodeoxynucleotide (ODN), made from the available sequence of gp43, a decrease in the fungus dissemination was observed. Results herein described suggest that genomic DNA from Rb. could have a immunostimulatory function as a Th-1-promoting adjuvant in susceptible mice.
Idioma Inglês
Data 2001-10-01
Publicado em Scandinavian Journal of Immunology. Oxford: Blackwell Science Ltd, v. 54, n. 4, p. 348-356, 2001.
ISSN 0300-9475 (Sherpa/Romeo, fator de impacto)
Editor Blackwell Science Ltd
Extensão 348-356
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000171128100002

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