Mouse B-1 cell-derived mononuclear phagocyte, a novel cellular component of acute non-specific inflammatory exudate

Mouse B-1 cell-derived mononuclear phagocyte, a novel cellular component of acute non-specific inflammatory exudate

Author Almeida, Sandro Rogério de Autor UNIFESP Google Scholar
Aroeira, L. S. Google Scholar
Frymuller, E. Autor UNIFESP Google Scholar
Dias, Maria Ângela Amorim Autor UNIFESP Google Scholar
Bogsan, Cristina Stewart Bittencourt Autor UNIFESP Google Scholar
Lopes, José Daniel Autor UNIFESP Google Scholar
Mariano, Mario Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Inst Nucl & Energet Res
Abstract At least three B cell subsets, B-1a, B-1b and B-2, or conventional B cells are present in the mouse periphery. Here we demonstrate that B-1 cells spontaneously proliferate in stationary cultures of normal adherent mouse peritoneal cells. B-1 cells were characterized by morphology, immunohistochemistry and flow cytometry. IgM was detected in the supernatants of these cultures. We demonstrated that the major cell population analyzed expresses the B-1b phenotype. When these cells were transferred to a new culture, a large proportion of them adhere to the plastic surface, and spread as bipolar cells endowed with the capacity to phagocytose via Fe and mannose receptors. Flow cytometry analysis of these adherent cells demonstrated that the great majority of them share both B-220 and Mac-1 antigens. Nevertheless, 45% of them were exclusively Mac-1(+). Finally, when they were labeled in vitro with [H-3]thymidine and transferred to the peritoneal cavity of naive mice, they migrate to a non-specific inflammatory focus induced by a foreign-body implant. These data demonstrate that B-1 cells, mainly B-1b cells, not only proliferate and differentiate into a mononuclear phagocyte in vitro, but also that they exit the peritoneal cavity and migrate to a non-specific inflammatory milieu.
Keywords inflammation
lymphocyte
macrophage
peritoneal cells
phagocyte
phagocytosis
Language English
Date 2001-09-01
Published in International Immunology. Oxford: Oxford Univ Press, v. 13, n. 9, p. 1193-1201, 2001.
ISSN 0953-8178 (Sherpa/Romeo, impact factor)
Publisher Oxford Univ Press
Extent 1193-1201
Origin http://dx.doi.org/10.1093/intimm/13.9.1193
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000171127200012
URI http://repositorio.unifesp.br/handle/11600/26613

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