Lack of association between N-ras gene mutations and clinical prognosis in Brazilian children with acute lymphoblastic leukemia

Lack of association between N-ras gene mutations and clinical prognosis in Brazilian children with acute lymphoblastic leukemia

Autor Clementino, NCD Google Scholar
Yamamoto, M. Google Scholar
Viana, M. B. Google Scholar
Figueiredo, M. S. Google Scholar
Kerbauy, J. Google Scholar
Saad, STO Google Scholar
Costa, F. F. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Point mutations in codons 12, 13 and 61 of the N-ras proto-oncogene have been detected in several human malignancies. We studied 170 patients with acute lymphoblastic leukemia (ALL), treated from 1988 to 1994 according to a protocol derived from BFM-83 studies, in order to evaluate the incidence and prognostic significance of mutations in this gene in childhood ALL. DNA was extracted from bone marrow smears at diagnosis and amplified by polymerase chain reaction (PCR). After screening with SSCP, PCR products were hybridized with allele specific probes and, in some cases, cloned in a pMOS Blue T vector and sequenced. Exon 2 was also studied in 101 children. Our results showed 4% of mutations in codons 12 and 13 and 2% in exon 2. Similar to a previous report, we identified 7% of mutations among children who were studied for both exons. A new mutation in codon 64 of the N-ras gene was detected in one patient. No significant clinical differences between patients with and without mutations were detected (sex, age, leukocyte counts at diagnosis, nutritional status, and risk factor according to the BFM protocol). Children with mutations in codons 12 and 13 showed significantly higher reactivity to PAS staining on blast cells than children with a wild type N-ras gene configuration. Comparison of overall- and recurrence-free survival did not show significant difference between groups with and without mutations. Our results suggest that mutations in the ras gene are infrequent in children with ALL at diagnosis and seem to be of low prognostic value.
Palavra-chave acute lymphoblastic leukaemia
N-ras gene mutations
Idioma Inglês
Data de publicação 2001-07-01
Publicado em Leukemia & Lymphoma. Reading: Harwood Acad Publ Gmbh, v. 42, n. 3, p. 473-479, 2001.
ISSN 1042-8194 (Sherpa/Romeo, fator de impacto)
Publicador Harwood Acad Publ Gmbh
Extensão 473-479
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000170255900023
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