Heparin and a cyclic octaphenol-octasulfonic acid (GL-522-Y-1) bind with high affinity to a 47-kDa protein from vascular endothelial cell surface and stimulate the synthesis and structural changes of heparan sulfate proteoglycan

Heparin and a cyclic octaphenol-octasulfonic acid (GL-522-Y-1) bind with high affinity to a 47-kDa protein from vascular endothelial cell surface and stimulate the synthesis and structural changes of heparan sulfate proteoglycan

Autor Pinhal, MAS Google Scholar
Trindade, E. S. Google Scholar
Fareed, J. Google Scholar
Dietrich, C. P. Google Scholar
Nader, H. B. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Loyola Med Sch
Resumo The effect of a cyclic octaphenol-octasulfonic acid (GL-522-Y-1), upon the synthesis of a heparan sulfate proteoglycan synthesized by endothelial cells (rabbit aorta and human umbilical vein) were studied. the cells were exposed to the compounds at various concentrations for different periods of time and the synthesized heparan sulfates analyzed by a combination of agarose gel electrophoresis and enzymatic degradation. the GL-522-Y-1, like heparin, change the sulfation pattern and stimulate two- to three-fold the synthesis of heparan sulfate proteoglycan secreted by rabbit and human endothelial cells in culture. GL-522-Y-1, besides being 100 times more active than heparin, also produces a significant enhancement of cell surface heparan sulfate in human vein endothelial cells. the effect of GL-522-Y-1 is completely abolished by methylation or acetylation of its free hydroxyl groups. Both heparin and GL-522-Y-1 have high affinity for a 47-kDa protein present at the surface of endothelial cells. These and other results lead us to speculate that the antithrombotic activity of heparin and GL522 in vivo could be related, at least in part, to the increased production of the heparan sulfate proteoglycan by endothelial cells. (C) 2001 Elsevier B.V. All rights reserved.
Palavra-chave heparan sulfate
human endothelial cells
rabbit endothelial cells
heparan sulfate synthesis
effect of heparin
effect of octaphenol-octasulfonic acid
effect of antithrombotics
Idioma Inglês
Data de publicação 2001-07-01
Publicado em Thrombosis Research. Oxford: Pergamon-Elsevier B.V., v. 103, n. 1, p. 35-45, 2001.
ISSN 0049-3848 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 35-45
Fonte http://dx.doi.org/10.1016/S0049-3848(01)00274-2
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000169594700006
Endereço permanente http://repositorio.unifesp.br/handle/11600/26590

Exibir registro completo




Arquivo

Arquivo Tamanho Formato Visualização

Não existem arquivos associados a este item.

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta