Toxoplasma gondii micronemal protein MIC1 is a lactose-binding lectin

Toxoplasma gondii micronemal protein MIC1 is a lactose-binding lectin

Autor Lourenco, E. V. Google Scholar
Pereira, Sandra R UNIFESP] Google Scholar
Faça, Vitor Marcel Autor UNIFESP Google Scholar
Coelho-Castelo, Arlete Aparecida Martins Google Scholar
Minéo, José R Google Scholar
Roque-Barreira, Maria Cristina Google Scholar
Greene, Lewis Joel Autor UNIFESP Google Scholar
Panunto-Castelo, Ademilson Google Scholar
Instituição Universidade de São Paulo (USP)
Universidade Federal de Uberlândia (UFU)
Universidade Federal de São Paulo (UNIFESP)
Resumo Host cell invasion by Toxoplasma gondii is a multistep process with one of the first steps being the apical release of micronemal proteins that interact with host receptors. We demonstrate here that micronemal protein 1 (MIC1) is a lactose-binding lectin, MIC1 and MIC4 were recovered in the lactose-eluted (Lac(+)) fraction on affinity chromatography on immobilized lactose of the soluble antigen fraction from tachyzoites of the virulent RH strain, MIC1 and MIC4 were both identified by N-terminal microsequencing. MIC4 was also identified by sequencing cDNA clones isolated from an expression library following screening with mouse polyclonal anti-60/70 kDa (Lac(+) proteins) serum. This antiserum localized the Lac(+) proteins on the apical region of T, gondii tachyzoites by confocal microscopy, the Lac(+) fraction induced hemagglutination (mainly type A human erythrocytes), which was inhibited by beta -galactosides (3 mM lactose and 12 mM galactose) but not by up to 100 mM melibiose (alpha -galactoside), fucose, mannose, or glucose or 0.2 mg/ml heparin, the lectin activity of the Lac(+) preparation was attributed to MIC1, because blotted MIC1, but not native MIC4, bound human erythrocyte type A and fetuin, the copurification of MIC1 and MIC4 may have been due to their association, as reported by others. These data suggest that MIC1 may act through its lectin activity during T, gondii infection.
Assunto Toxoplasma gondii
microneme
MIC 1
MIC4
lactose-binding lectin
Idioma Inglês
Data 2001-07-01
Publicado em Glycobiology. Cary: Oxford Univ Press Inc, v. 11, n. 7, p. 541-547, 2001.
ISSN 0959-6658 (Sherpa/Romeo, fator de impacto)
Editor Oxford Univ Press Inc
Extensão 541-547
Fonte http://dx.doi.org/10.1093/glycob/11.7.541
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000169850400004
URI http://repositorio.unifesp.br/handle/11600/26585

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