Cloning and characterization of a LON gene homologue from the human pathogen Paracoccidioides brasiliensis

Cloning and characterization of a LON gene homologue from the human pathogen Paracoccidioides brasiliensis

Autor Barros, Tânia Fraga Autor UNIFESP Google Scholar
Puccia, Rosana Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo A LON gene homologue from the human pathogen Paracoccidioides brasiliensis (PbLON) has been cloned, sequenced and characterized. It encodes a putative ATP-dependent proteinase Lon, which in Saccharomyces cerevisisae (PIM1) is a heat-inducible protein involved in the degradation of abnormal or short-lived proteins in the mitochondria. the PbLON ORF is within a 3369 bp fragment interrupted by two introns located in the 3 ' segment. the 5 ' and 3 ' regions flanking the ORF contain sequences which resemble known transcription elements. Several transcription binding factor motifs have also been found, including sites for heat shock/stress response and nitrogen control, the deduced protein consists of 1063 residues containing a mitochondrial import signal at the N-terminus and conserved ATP-binding (GPPGVGKT) and serine catalytic (KDGPSAG) sites. It shares high identity with Lon homologues from S. cerevisiae (73%), Homo sapiens (62%) and Escherichia coli (56%). in P. brasiliensis, an MDJ1 putative gene has also been partially sequenced adjacent to PbLON, possibly sharing divergently orientated promoter elements, This chromosomal organization is interesting, since Mdj1p is a heat shock chaperone essential for substrate degradation by PIM1 in yeast. the LON nucleotide and flanking sequence regions have been submitted to GenBank under Accession No. AF239178, Copyright (C) 2001 John Wiley & Sons, Ltd.
Palavra-chave LON homologue
Paracoccidiodes brasiliensis
heat shock protein
ATP-dependent proteinase
MDJ1 homologue
Idioma Inglês
Data de publicação 2001-07-01
Publicado em Yeast. W Sussex: John Wiley & Sons Ltd, v. 18, n. 10, p. 981-988, 2001.
ISSN 0749-503X (Sherpa/Romeo, fator de impacto)
Publicador Wiley-Blackwell
Extensão 981-988
Fonte http://dx.doi.org/10.1002/yea.745
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000169965500011
Endereço permanente http://repositorio.unifesp.br/handle/11600/26584

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