Interactions of Escherichia coli strains of non-EPEC serogroups that carry eae and lack the EAF and stx gene sequences with undifferentiated and differentiated intestinal human Caco-2 cells

Interactions of Escherichia coli strains of non-EPEC serogroups that carry eae and lack the EAF and stx gene sequences with undifferentiated and differentiated intestinal human Caco-2 cells

Autor Rosa, Ana CP Google Scholar
Vieira, Monica AM Autor UNIFESP Google Scholar
Tibana, Anita Google Scholar
Gomes, Tania Aparecida Tardelli Autor UNIFESP Google Scholar
Andrade, João RC Google Scholar
Instituição Universidade do Estado do Rio de Janeiro (UERJ)
Universidade Federal de São Paulo (UNIFESP)
Universidade Federal do Rio de Janeiro (UFRJ)
Resumo Escherichia coli strains of non-EPEC serotypes that carry eae and lack the EAF and the Shiga toxin (sts) gene sequences have been found in acute diarrhea. Both the cell association and the cell entry of these strains in human intestinal epithelial cells were studied as a function of cell differentiation and polarization. the eae+/EAF-/stx- non-EPEC E. coli strains invaded undifferentiated Caco-2 cells more efficiently than differentiated cells. in contrast, prototype EPEC strain E2348/69 did not show significative differences from invasion rates of undifferentiated and differentiated cells. the uptake of these strains was greatly enhanced by pretreatment of differentiated Caco-2 cells with EGTA. These results suggest that the eae+/EAF-/stx-non-EPEC E. coli invasion of intestinal cells may be dependent on receptors expressed on the surface of undifferentiated cells and the basolateral pole of differentiated cells. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.
Palavra-chave Caco-2 cell
enterocyte differentiation
bacterial adherence
bacterial invasion
eae plus /EAF-/stx Escherichia coli
Idioma Inglês
Data de publicação 2001-06-12
Publicado em Fems Microbiology Letters. Amsterdam: Elsevier B.V., v. 200, n. 1, p. 117-122, 2001.
ISSN 0378-1097 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 117-122
Fonte http://dx.doi.org/10.1111/j.1574-6968.2001.tb10702.x
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000169419900019
Endereço permanente http://repositorio.unifesp.br/handle/11600/26582

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