In vivo characterization of Lopap, a prothrombin activator serine protease from the Lonomia obliqua caterpillar venom

In vivo characterization of Lopap, a prothrombin activator serine protease from the Lonomia obliqua caterpillar venom

Autor Reis, C. V. Google Scholar
Farsky, SHP Google Scholar
Fernandes, B. L. Google Scholar
Santoro, M. L. Google Scholar
Oliva, MLV Google Scholar
Mariano, M. Google Scholar
Chudzinski-Tavassi, A. M. Google Scholar
Instituição FAPESP
Universidade de São Paulo (USP)
Butantan Inst
Universidade Federal de São Paulo (UNIFESP)
Resumo Increasing occurrence of hemorrhagic syndrome in man, caused by contact with Lonomia obliqua caterpillars, has been reported in Southern Brazil in the past 10 years. the L. obliqua venom causes a severe consumptive coagulopathy, which can lead to hemorrhagic syndrome. L. obliqua prothrombin activator protease (Lopap) is a 69-kDa prothrombin activator serine protease isolated from L. obliqua caterpillar bristle extract, which is able to evoke thrombus formation, unclottable blood, and fibrinogen depletion when injected into the blood stream of rats. the purified protein generated thrombin from prothrombin, able to clot purified human fibrinogen and plasma. A decrease in platelet count and inhibition of collagen-induced platelet aggregation were observed, as well as leukocyte infiltration in the lungs. Ln addition, we observed congestion and hemorrhage in renal glomeruli and necrosis in renal distal tubules. These data support the hypothesis that Lopap contributes to the clinical syndrome caused by human contact with L. obliqua, most probably through prothrombin activation, resulting in a consumption coagulopathy. (C) 2001 Elsevier B.V. All rights reserved.
Palavra-chave Lonomia obliqua
caterpillar
prothrombin activator
coagulopathy
Idioma Inglês
Data de publicação 2001-06-01
Publicado em Thrombosis Research. Oxford: Pergamon-Elsevier B.V., v. 102, n. 5, p. 437-443, 2001.
ISSN 0049-3848 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 437-443
Fonte http://dx.doi.org/10.1016/S0049-3848(01)00268-7
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000169278900007
Endereço permanente http://repositorio.unifesp.br/handle/11600/26570

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