Comparative EPR and fluorescence conformational studies of fully active spin-labeled melanotropic peptides

Comparative EPR and fluorescence conformational studies of fully active spin-labeled melanotropic peptides

Author Nakaie, Clovis Ryuichi Autor UNIFESP Google Scholar
Barbosa, Simone Reis Autor UNIFESP Google Scholar
Vieira, Renata de Freitas Fisher Autor UNIFESP Google Scholar
Fernandez, R. M. Google Scholar
Cilli, Eduardo Maffud Autor UNIFESP Google Scholar
Castrucci, Ana Maria de Lauro Google Scholar
Visconti, Maria Aparecida Google Scholar
Ito, A. S. Google Scholar
Lamy-Freund, M. T. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Similar to melanocyte stimulating hormone (alpha -MSH), its potent and long-acting analogue, [Nle(4), D-Phe(7)]alpha -MSH, when labeled with the paramagnetic amino acid probe 2,2,6,6-tetramethylpiperidine-N-oxyl-4-amino-4-carboxylic acid (Toac), maintains its full biological potency, thus validating any comparative structural investigations between the two labeled peptides, Correlation times, calculated from tire electron paramagnetic resonance signal of Toac bound to the peptides, and Toac-Trp distances, estimated from the Toac fluorescence quenching of the Trp residue present in the peptides, indicate a more rigid and folded structure for the potent analogue as compared to the hormone, in aqueous medium. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
Keywords peptide
alpha-melanocyte stimulating hormone
electron paramagnetic resonance
2,2,6,6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid
fluorescence
spin label
Language English
Date 2001-05-25
Published in Febs Letters. Amsterdam: Elsevier B.V., v. 497, n. 2-3, p. 103-107, 2001.
ISSN 0014-5793 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 103-107
Origin http://dx.doi.org/10.1016/S0014-5793(01)02449-8
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000169120900009
URI http://repositorio.unifesp.br/handle/11600/26557

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