Comparison of different delivery systems of vaccination for the induction of protection against tuberculosis in mice

Comparison of different delivery systems of vaccination for the induction of protection against tuberculosis in mice

Autor Lima, K. M. Google Scholar
Bonato, VLD Google Scholar
Faccioli, L. H. Google Scholar
Brandao, I. T. Google Scholar
Santos, S. A. dos Google Scholar
Coelho-Castelo, AAM Google Scholar
Leao, S. C. Google Scholar
Silva, C. L. Google Scholar
Instituição Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Resumo The way to deliver antigens and cellular requirements for long-lasting protection against tuberculosis are not known. Immunizations with mycobacterial 65 kDa heat shock protein (hsp65) expressed from J774-hsp65 cells (antigen-presenting cells that endogenously produce hsp65 antigen) or from plasmid DNA, or with the protein entrapped in cationic liposomes. can each give protective immunity similar to that obtained from live Bacillus Calmette Guerin (BCG), whereas injecting the protein in Freund's incomplete adjuvant (FIA) has minimal effect. Protective procedures elicited high frequencies of antigen-reactive rp T cells with CD4(+)/CD8(-) and CD8(+)/CD4(-) phenotypes. Protection correlated with the abundance of hsp65-dependent cytotoxic CD8(+)/CD4(-)/CD44(hi) cells. the frequency of these cells and the level of protection declined during 8 months after J774-hsp65 or liposome-mediated immunization with hsp65 protein but were sustained or steadily increased over this period after hsp65-DNA or BCG immunizations. IFN-gamma predominated over IL-4 among the hsp65-reactive CD8(+)/CD4(-) and CD4+/CD8(-) populations after J774-hsp65-, hsp65-liposome-, and hsp65-DNA-mediated immunizations, but similar levels of these cytokines prevailed after BCG vaccination. (C) 2001 Elsevier B.V. All rights reserved.
Palavra-chave DNA vaccination
liposomes
transfected macrophages
tuberculosis BCG
cytokines
Idioma Inglês
Data de publicação 2001-05-14
Publicado em Vaccine. Oxford: Elsevier B.V., v. 19, n. 25-26, p. 3518-3525, 2001.
ISSN 0264-410X (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 3518-3525
Fonte http://dx.doi.org/10.1016/S0264-410X(01)00042-1
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000168992600025
Endereço permanente http://repositorio.unifesp.br/handle/11600/26551

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