Effect of temperature on peptide chain aggregation: an EPR study of model peptidyl-resins

Effect of temperature on peptide chain aggregation: an EPR study of model peptidyl-resins

Autor Ribeiro, SCF Google Scholar
Schreier, S. Google Scholar
Nakaie, C. R. Google Scholar
Cilli, E. M. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Resumo The effect of temperature on the dynamics of peptide chains inside resin beads was monitored by electron paramagnetic resonance (EPR) spectroscopy. A. two-component spectra was obtained for low and highly peptide-loaded model peptidyl-resins labeled with the paramagnetic aminoacid 2,2,6,6-tetramethyl-piperidine-N-oxyl-4-amino-4-carboxylic acid (TOAC), indicating the presence of strongly and weakly immobilized populations. Increasing levels of chain disaggregation were observed with increasing temperature, leading in some cases to a complete disappearance of the more immobilized population. the present findings demonstrate that EPR spectral parameters are highly sensitive to the solvation properties of labeled sites inside the resin matrix and can be of great value for the understanding of polymer-supported processes or reactions. (C) 2001 Elsevier B.V. All rights reserved.
Idioma Inglês
Data de publicação 2001-05-07
Publicado em Tetrahedron Letters. Oxford: Pergamon-Elsevier B.V., v. 42, n. 19, p. 3243-3246, 2001.
ISSN 0040-4039 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 3243-3246
Fonte http://dx.doi.org/10.1016/S0040-4039(01)00414-2
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000168404000001
Endereço permanente http://repositorio.unifesp.br/handle/11600/26550

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