In vivo and in vitro cytotoxicity of brown spider venom for blood vessel endothelial cells

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dc.contributor.author Veiga, S. S.
dc.contributor.author Zanetti, V. C.
dc.contributor.author Franco, CRC
dc.contributor.author Trindade, E. S.
dc.contributor.author Porcionatto, M. A.
dc.contributor.author Mangili, O. C.
dc.contributor.author Gremski, W.
dc.contributor.author Dietrich, C. P.
dc.contributor.author Nader, H. B.
dc.date.accessioned 2016-01-24T12:31:22Z
dc.date.available 2016-01-24T12:31:22Z
dc.date.issued 2001-05-01
dc.identifier http://dx.doi.org/10.1016/S0049-3848(01)00254-7
dc.identifier.citation Thrombosis Research. Oxford: Pergamon-Elsevier B.V., v. 102, n. 3, p. 229-237, 2001.
dc.identifier.issn 0049-3848
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/26541
dc.description.abstract The effect of brown spider (Loxosceles intermedia) venom on endothelial cells was investigated in vivo and in vitro. Morphological and ultrastructural observations by light microscopy and transmission electron microscopy showed that the venom acts in vivo upon vessel endothelial cells of rabbits that were intradermally injected, evoking vessel instability, cytoplasmic endothelial cell vacuolization, and blebs. Likewise, treatment of rabbit endothelial cells in culture with the venom led to loss of adhesion of the cells to the substrate. Endothelial cells in culture were metabolically radiolabeled with sodium [S-35]- sulfate and the sulfated compounds (proteoglycans and sulfated proteins) from medium, cell surface, and extracellular matrix (ECM) were analyzed. Agarose gel electrophoresis and SDS-PAGE showed that the venom is active on the ECM and on cell surface proteoglycans, shedding these molecules into the culture medium. in addition, when purified heparan sulfate proteoglycan (HSPG) and purified laminin-entactin (LN/ET) complex were incubated with the venom we observed a partial degradation of the protein core of HSPG as well as the hydrolysis of entactin. the above results suggest that the L. intermedia venom has a deleterious effect on the endothelium of vessels both in vivo and in culture, removing important constituents such as HSPG and entactin that are involved in the adhesion of endothelial cells and of subendothelial ECM organization. (C) 2001 Elsevier B.V. All rights reserved. en
dc.format.extent 229-237
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Thrombosis Research
dc.rights Acesso restrito
dc.subject brown spider en
dc.subject venom en
dc.subject blood vessel en
dc.subject endothelial cells en
dc.title In vivo and in vitro cytotoxicity of brown spider venom for blood vessel endothelial cells en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Univ Fed Parana
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ Fed Parana, Dept Cell Biol, BR-81531990 Curitiba, Parana, Brazil
dc.description.affiliation Univ Fed Parana, Dept Physiol, BR-80060000 Curitiba, Parana, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Biochem, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Biochem, São Paulo, Brazil
dc.identifier.doi 10.1016/S0049-3848(01)00254-7
dc.description.source Web of Science
dc.identifier.wos WOS:000168734800006



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