In vivo and in vitro cytotoxicity of brown spider venom for blood vessel endothelial cells

In vivo and in vitro cytotoxicity of brown spider venom for blood vessel endothelial cells

Autor Veiga, S. S. Google Scholar
Zanetti, V. C. Google Scholar
Franco, CRC Google Scholar
Trindade, E. S. Google Scholar
Porcionatto, M. A. Google Scholar
Mangili, O. C. Google Scholar
Gremski, W. Google Scholar
Dietrich, C. P. Google Scholar
Nader, H. B. Google Scholar
Instituição Univ Fed Parana
Universidade Federal de São Paulo (UNIFESP)
Resumo The effect of brown spider (Loxosceles intermedia) venom on endothelial cells was investigated in vivo and in vitro. Morphological and ultrastructural observations by light microscopy and transmission electron microscopy showed that the venom acts in vivo upon vessel endothelial cells of rabbits that were intradermally injected, evoking vessel instability, cytoplasmic endothelial cell vacuolization, and blebs. Likewise, treatment of rabbit endothelial cells in culture with the venom led to loss of adhesion of the cells to the substrate. Endothelial cells in culture were metabolically radiolabeled with sodium [S-35]- sulfate and the sulfated compounds (proteoglycans and sulfated proteins) from medium, cell surface, and extracellular matrix (ECM) were analyzed. Agarose gel electrophoresis and SDS-PAGE showed that the venom is active on the ECM and on cell surface proteoglycans, shedding these molecules into the culture medium. in addition, when purified heparan sulfate proteoglycan (HSPG) and purified laminin-entactin (LN/ET) complex were incubated with the venom we observed a partial degradation of the protein core of HSPG as well as the hydrolysis of entactin. the above results suggest that the L. intermedia venom has a deleterious effect on the endothelium of vessels both in vivo and in culture, removing important constituents such as HSPG and entactin that are involved in the adhesion of endothelial cells and of subendothelial ECM organization. (C) 2001 Elsevier B.V. All rights reserved.
Palavra-chave brown spider
venom
blood vessel
endothelial cells
Idioma Inglês
Data de publicação 2001-05-01
Publicado em Thrombosis Research. Oxford: Pergamon-Elsevier B.V., v. 102, n. 3, p. 229-237, 2001.
ISSN 0049-3848 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 229-237
Fonte http://dx.doi.org/10.1016/S0049-3848(01)00254-7
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000168734800006
Endereço permanente http://repositorio.unifesp.br/handle/11600/26541

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