Blockade by agmatine of catecholamine release from chromaffin cells is unrelated to imidazoline receptors

Blockade by agmatine of catecholamine release from chromaffin cells is unrelated to imidazoline receptors

Autor Santos, W. C. Google Scholar
Hernandez-Guijo, J. M. Google Scholar
Ruiz-Nuno, A. Google Scholar
Olivares, R. Google Scholar
Jurkiewicz, A. Google Scholar
Gandia, L. Google Scholar
Garcia, A. G. Google Scholar
Instituição Univ Autonoma Madrid
Universidade Federal de São Paulo (UNIFESP)
Univ Amazonas
Hosp Princesa
Resumo The blockade of exocytosis induced by the putative endogenous ligand for imidazoline receptors, agmatine, was studied by using on-line measurement of catecholamine release in bovine adrenal medullary chromaffin cells. Agmatine inhibited the acetylcholine-evoked release of catecholamines in a concentration-dependent manner (IC50 = 366 muM); the K+-evoked release of catecholamines was unaffected. Clonidine (100 muM) and moxonidine (100 muM) also inhibited by 75% and 50%, respectively, the acetylcholine-evoked response. in cells voltage-clamped at -80 mV, the intermittent application of acetylcholine pulses elicited whole-cell inward currents (I-ACh) that were blocked 63% by 1 mM agmatine. the onset of blockade was very fast (tau (on) = 31 ms); the recovery of the current after washout of agmatine also occurred very rapidly (tau (off) = 39 ms). Efaroxan (10 muM) did not affect the inhibition of I-ACh elicited by 1 mM agmatine. I-ACh was blocked 90% by 100 muM clonidine and 50% by 100 muM moxonidine. the concentration-response curve for acetylcholine to elicit inward currents was shifted to the right in a non-parallel manner by 300 muM agmatine. the blockade of I-ACh caused by agmatine (100 muM) was similar at various holding potentials, around 50%. When intracellularly applied, agmatine did not block I-ACh. At 1 mM, agmatine blocked I-Na by 23%, I-Ba by 14%, I-K(Ca) by 16%, and I-K(VD) by 18%. in conclusion, agmatine blocks exocytosis in chromaffin cells by blocking nicotinic acetylcholine receptor currents. in contrast to previous views, these effects seem to be unrelated to imidazoline receptors. (C) 2001 Elsevier Science B.V. All rights reserved.
Assunto agmatine
nicotinic receptor
Ca2+ channel
chromaffin cell
Idioma Inglês
Data 2001-04-06
Publicado em European Journal of Pharmacology. Amsterdam: Elsevier B.V., v. 417, n. 1-2, p. 99-109, 2001.
ISSN 0014-2999 (Sherpa/Romeo, fator de impacto)
Editor Elsevier B.V.
Extensão 99-109
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000168220400012

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