Preliminary crystallographic studies of EcTI, a serine proteinase inhibitor from Enterolobium contortisiliquum seeds

Preliminary crystallographic studies of EcTI, a serine proteinase inhibitor from Enterolobium contortisiliquum seeds

Autor Batista, IFC Google Scholar
Nonato, M. C. Google Scholar
Bonfadini, M. R. Google Scholar
Beltramini, L. M. Google Scholar
Oliva, MLV Google Scholar
Sampaio, M. U. Google Scholar
Sampaio, CAM Google Scholar
Garratt, R. C. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Resumo Enterolobium contortisiliquum trypsin inhibitor (EcTI) belongs to the Kunitz family of plant inhibitors, which are widely distributed in nature, especially in plant seeds. EcTI is composed of two polypeptide chains with a total of 174 residues, homologous to other inhibitors from the same family. EcTI crystals, which were obtained with the acupuncture-gel technique, diffract to 2.0 Angstrom resolution and belong to space group P2(1), with unit-cell parameters a = 37.12, b = 38.42, c = 54.08 Angstrom, beta = 98.08 degrees. Molecular-replacement techniques using Erythrina caffra trypsin inhibitor (PDB code 1tie) as the search model indicate one monomer in the asymmetric unit. the secondary-structure content of EcTI was determined by circular dichroism spectroscopy, yielding values compatible with the expected topology.
Idioma Inglês
Data de publicação 2001-04-01
Publicado em Acta Crystallographica Section D-biological Crystallography. Copenhagen: Munksgaard Int Publ Ltd, v. 57, p. 602-604, 2001.
ISSN 0907-4449 (Sherpa/Romeo, fator de impacto)
Publicador Munksgaard Int Publ Ltd
Extensão 602-604
Fonte http://dx.doi.org/10.1107/S0907444901001810
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000167663500021
Endereço permanente http://repositorio.unifesp.br/handle/11600/26524

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