Monitoring of intragraft and peripheral blood TIRC7 expression as a diagnostic tool for acute cardiac rejection in humans

Monitoring of intragraft and peripheral blood TIRC7 expression as a diagnostic tool for acute cardiac rejection in humans

Autor Shulzhenko, N. Google Scholar
Morgun, A. Google Scholar
Rampim, G. F. Google Scholar
Franco, M. Google Scholar
Almeida, D. R. Google Scholar
Diniz, RVZ Google Scholar
Carvalho, ACC Google Scholar
Gerbase-DeLima, M. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo T-cell immune response cDNA 7 (TIRC7) is a recently described T-cell costimulatory molecule that exhibits a central role in T-cell activation in vitro and in vivo. the present study was undertaken to investigate association between intragraft and peripheral blood mononuclear cell (PBMC) TIRC7 mRNA levels and cardiac allograft rejection in humans. TIRC7 gene expression levels were determined by a quantitative-competitive reverse transcriptase-polymerase chain reaction (QC-RT-PCR) in endomyocardial biopsies and in PBMC from cardiac transplant recipients. Biopsies collected during rejection or up to IS days before rejection showed heightened TIRC7 mRNA expression in comparison with biopsies without rejection. All prerejection and rejection biopsies showed TIRC7 mRNA upregulation, while this was present in only 30% of the biopsies without rejection. Regarding TIRC7 mRNA in PBMC, transplant recipients showed lower levels than healthy individuals and, in contrast to the results obtained in biopsies, the levels were lower during rejection than in rejection-free periods. in summary, TIRC7 mRNA expression levels increase in biopsies and decrease in peripheral blood during acute cardiac rejection. We conclude chat intragraft detection of TIRC7 transcripts is a useful tool not only for the diagnosis but also for the prediction of acute heart allograft rejection episodes. (C) American Society for Histocompatibility and Immunogenetics. 2001. Published by Elsevier Science Inc.
Assunto TIRC7
cardiac allograft rejection
immunologic monitoring
gene activation
Idioma Inglês
Data 2001-04-01
Publicado em Human Immunology. New York: Elsevier B.V., v. 62, n. 4, p. 342-347, 2001.
ISSN 0198-8859 (Sherpa/Romeo, fator de impacto)
Editor Elsevier B.V.
Extensão 342-347
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000168062900005

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