Mineralized dermal layer of the Brazilian tree-frog Corythomantis greeningi

Mineralized dermal layer of the Brazilian tree-frog Corythomantis greeningi

Author Katchburian, E. Google Scholar
Antoniazzi, M. M. Google Scholar
Jared, C. Google Scholar
Faria, F. P. Google Scholar
Santos, H. S. Google Scholar
Freymuller, E. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Inst Butantan
Universidade de São Paulo (USP)
Abstract Some species of anuran amphibians possess a calcified dermal layer (the Eberth-Kastschenko layer) located between the stratum spongiosum and the stratum compactum. This layer consists of calcium phosphate deposits, proteoglycans, and glycosaminoglycans. Although regarded as a protective layer against desiccation, a calcium reservoir, or possibly a remnant of a dermal skeleton present in anuran ancestors, very little is known about its origin, structure, and function. Thus, we studied the structure and composition of the mineralized dermal layer of Corythomantis greeningi, a peculiar hylid from the Brazilian semiarid region (caatinga), using conventional and cryosubstitution methods combined with transmission, scanning, and analytical electron microscopy. Results show that the dermal layer consists of dense, closely juxtaposed, globular structures. Although the electron opacity of the globules was variable, depending on the type of preparation, crystal-like inclusions were present in all of them, as confirmed by dark field microscopy. Electron probe X-ray microanalysis showed calcium, phosphorus, and oxygen, and electron diffraction revealed a crystalline structure comparable to that of a hydroxyapatite. (C) 2001 Wiley-Liss, Inc.
Keywords amphibia
skin
calcified dermal layer
Corythomantis greeningi
biomineralization
Language English
Date 2001-04-01
Published in Journal of Morphology. New York: Wiley-liss, v. 248, n. 1, p. 56-63, 2001.
ISSN 0362-2525 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 56-63
Origin http://dx.doi.org/10.1002/jmor.1020
Access rights Closed access
Type Article
Web of Science ID WOS:000167678100004
URI http://repositorio.unifesp.br/handle/11600/26515

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