Phenotypic and genotypic characteristics of Escherichia coli strains of non-enteropathogenic E-coli (EPEC) serogroups that carry eae and lack the EPEC adherence factor and shiga toxin DNA probe sequences

Phenotypic and genotypic characteristics of Escherichia coli strains of non-enteropathogenic E-coli (EPEC) serogroups that carry eae and lack the EPEC adherence factor and shiga toxin DNA probe sequences

Author Vieira, Monica Aparecida Midolli Autor UNIFESP Google Scholar
Andrade, JRC Google Scholar
Trabulsi, L. R. Google Scholar
Rosa, ACP Google Scholar
Dias, AMG Google Scholar
Ramos, SRTS Google Scholar
Frankel, G. Google Scholar
Gomes, Tania Aparecida Tardelli Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Universidade do Estado do Rio de Janeiro (UERJ)
Univ London Imperial Coll Sci Technol & Med
Abstract This study was conducted to characterize the virulence potential of 59 Escherichia coli strains carrying eae and lacking the enteropathogenic E. coli adherence factor and Shiga toxin probe sequences. in hybridization studies, all strains carried the locus of enterocyte effacement (LEE)-associated DNA sequences. of the other 15 virulence DNA sequences tested, hly was the most frequent (44.1%); 17 combinations of these sequences were found, but strains carrying eae only (eae profile) were the most frequent (35.6%). Except for 1 cytodetaching strain, all others adhered to HeLa and Caco-2 cells, most of which (similar to 75.0%) showed variations of the localized adherence pattern. Actin accumulation was detected in 75.9% of the nondetaching strains. Most strains had LEE, probably inserted in pheU (49.2%), and presented a nontypeable intimin (83.1%). Translocated intimin receptor-derived DNA sequences correlated with enteropathogenic and enterohemorrhagic E. coli in 61.0% and 32.0% of the strains, respectively. Thirty-five different serotypes were found. Only strains with the eae profile were associated with diarrhea (P = .039).
Language English
Date 2001-03-01
Published in Journal of Infectious Diseases. Chicago: Univ Chicago Press, v. 183, n. 5, p. 762-772, 2001.
ISSN 0022-1899 (Sherpa/Romeo, impact factor)
Publisher Univ Chicago Press
Extent 762-772
Origin http://dx.doi.org/10.1086/318821
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000166836500011
URI http://repositorio.unifesp.br/handle/11600/26495

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