Decreased lipolysis and enhanced glycerol and glucose utilization by adipose tissue prior to development of obesity in monosodium glutamate (MSG) treated-rats

Decreased lipolysis and enhanced glycerol and glucose utilization by adipose tissue prior to development of obesity in monosodium glutamate (MSG) treated-rats

Autor Dolnikoff, Miriam Autor UNIFESP Google Scholar
Martin-Hidalgo, A. Google Scholar
Machado, U. F. Google Scholar
Lima, F. B. Google Scholar
Herrera, E. Google Scholar
Instituição Univ San Pablo CEU
Universidade Federal de São Paulo (UNIFESP)
Hosp Ramon y Cajal
Universidade de São Paulo (USP)
Resumo OBJECTIVE: To determine the metabolic alterations that lead to the neonatal administration of monosodium glutamate (MSG), which results in arrested growth and obesity.ANIMALS and DESIGN: Wistar rats were injected 5 times, every other day, with 4 g of MSG/kg b.w. or with hyperosmotic saline (controls), within the first 10 days of life, and were studied at the age of 30 days.RESULTS: Body weight was lower, whereas adipocyte lipid content, cell diameter, surface area and volume were higher in MSG rats than in controls. Plasma glucose, insulin, NEFA, glycerol and triglyceride levels, and in vitro production of NEFA by lumbar fat pad pieces incubated under basal conditions or in the presence of epinephrine and epinephrine plus glucose in the media were lower in MSG than in control rats. in the same fat pad pieces, the conversion of 1-C-14-glycerol into fatty acids was always enhanced and its conversion into glyceride glycerol was enhanced when incubations were carried out in the presence of epinephrine or glucose. Both the hormone sensitive lipase activity and mRNA expression were lower in adipose tissue from MSG rats. Besides, the number of insulin receptors, lipid synthesis from (UC)-C-14 glucose, H-3-2-deoxy D-glucose uptake and cellular GLUT4 translocation index were higher in adipocytes from MSG rats than from the controls.CONCLUSION: It is proposed that an enhanced insulin sensitivity in 1 month old MSG rats is responsible for the decreased lipolytic activity and enhanced glucose uptake. in addition, the enhanced lipogenesis and glycerol reutilization seen in their adipose tissue, disturbs the normal balance between fat depots breakdown and accumulation in favor of the latter.
Palavra-chave monosodium glutamate obesity
lipolysis
hormone sensitive lipase
glycerol
GLUT4
Idioma Inglês
Data de publicação 2001-03-01
Publicado em International Journal of Obesity. Basingstoke: Nature Publishing Group, v. 25, n. 3, p. 426-433, 2001.
ISSN 0307-0565 (Sherpa/Romeo, fator de impacto)
Publicador Nature Publishing Group
Extensão 426-433
Fonte http://dx.doi.org/10.1038/sj.ijo.0801517
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000167206400018
Endereço permanente http://repositorio.unifesp.br/handle/11600/26492

Exibir registro completo




Arquivo

Arquivo Tamanho Formato Visualização

Não existem arquivos associados a este item.

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta