Characterization of monoclonal antibody MEST-2 specific to glucosylceramide of fungi and plants

Characterization of monoclonal antibody MEST-2 specific to glucosylceramide of fungi and plants

Autor Toledo, Marcos Sergio de Autor UNIFESP Google Scholar
Suzuki, Erika Autor UNIFESP Google Scholar
Levery, SB Google Scholar
Straus, Anita Hilda Autor UNIFESP Google Scholar
Takahashi, Helio Kiyoshi Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Univ Georgia
Resumo An IgG2a monoclonal antibody anti-glucosylceramide was established and termed MEST-2. High performance thin layer chromatography immunostaining, and solid-phase radioimmunoassay showed that MEST-2 reacts with glucosylceramide from yeast and mycelium forms of Paracoccidioides brasiliensis, Histoplasma capsulatum, and Sporothrix schenckii; from hyphae of Aspergillus fumigatus; and from yeast forms of Candida albicans, Cryptococcus neoformans, Cryptococcus laurentii, and Cryptococcus albidus, Studies on the fine specificity of MEST-2 showed that it recognizes the beta -D-glucose residue, and that the 2-hydroxy group present in the fatty acid is an important auxiliary feature for the antibody binding. It was also demonstrated that phosphatidylcholine and ergosterol modulate MEST-2 reactivity to glucosylceramide, by solid-phase radioimmunoassay. Indirect immunofluorescence showed that MEST-2 reacts with the surface of yeast forms of P. brasiliensis, H. capsulatum and S. schenckii. Weak staining of mycelial forms of P, brasiliensis and hyphae of A. fumigatus was also observed. the availability of a monoclonal antibody specific to fungal glucosylceramide, and its potential use in analyzing biological roles attributed to glucosylceramide in fungi are discussed.
Palavra-chave 2-hydroxy fatty acid
glucosylceramide
monoclonal antibody
paracoccidioides brasiliensis
pathogenic fungi
Idioma Inglês
Data de publicação 2001-02-01
Publicado em Glycobiology. Cary: Oxford Univ Press Inc, v. 11, n. 2, p. 105-112, 2001.
ISSN 0959-6658 (Sherpa/Romeo, fator de impacto)
Publicador Oxford Univ Press Inc
Extensão 105-112
Fonte http://dx.doi.org/10.1093/glycob/11.2.105
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000167957700004
Endereço permanente http://repositorio.unifesp.br/handle/11600/26477

Exibir registro completo




Arquivo

Arquivo Tamanho Formato Visualização

Não existem arquivos associados a este item.

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta