Phosphatidylinositol-specific phospholipase C (PI-PLC) cleavage of GPI-anchored surface molecules of Trypanosoma cruzi triggers in vitro morphological reorganization of trypomastigotes

Phosphatidylinositol-specific phospholipase C (PI-PLC) cleavage of GPI-anchored surface molecules of Trypanosoma cruzi triggers in vitro morphological reorganization of trypomastigotes

Autor Mortara, R. A. Google Scholar
Minelli, LMS Google Scholar
Vandekerchove, F. Google Scholar
Nussenzweig, V Google Scholar
Ramalho-Pinto, F. J. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
NYU Med Ctr
Resumo Trypanosoma cruzi trypomastigotes treated with phosphatidylinositol-specific phospholipase C (PI-PLC) in vitro are rapidly induced to differentiate into round forms. Using confocal microscopy, we were able to show that trypomastigotes treated with PI-PLC initiate the process of flagellum remodeling by 30 sec after contact with the enzyme and amastigote-like forms are detected as early as 10 min after PI-PLC treatment. Scanning and transmission electron microscopy indicate that trypomastigotes undergo a previously undescribed process of flagellum circularization and internalization. Analysis of the flagellar complex with monoclonal antibody 4D9 shows heterogeneous labeling among the parasites, suggesting a remodeling of these molecules. After PI-PLC treatment, parasites rapidly lose the surface marker Ssp-3 and 24 h post-treatment they begin to exhibit a circular nucleus and a rod-shaped kinetoplast. By flow cytometry analysis and confocal microscopy, the Ssp-4 amastigote-specific epitope can be detected on the parasite surface. This indicates that thr release of trypomastigote GPI-anchored molecules by exogenous PI-PLC in vitro can trigger morphological changes.
Palavra-chave confocal microscopy
flagellum
GPI
PI-PLC
protozoa
Trypanosoma cruzi
trypomastigote
Idioma Inglês
Data de publicação 2001-01-01
Publicado em Journal of Eukaryotic Microbiology. Lawrence: Soc Protozoologists, v. 48, n. 1, p. 27-37, 2001.
ISSN 1066-5234 (Sherpa/Romeo, fator de impacto)
Publicador Soc Protozoologists
Extensão 27-37
Fonte http://dx.doi.org/10.1111/j.1550-7408.2001.tb00413.x
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000167286400007
Endereço permanente http://repositorio.unifesp.br/handle/11600/26460

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