Infection of Vero cells with Coxiella burnetii phase II: relative intracellular bacterial load and distribution estimated by confocal laser scanning microscopy and morphometry

Infection of Vero cells with Coxiella burnetii phase II: relative intracellular bacterial load and distribution estimated by confocal laser scanning microscopy and morphometry

Autor Zamboni, D. S. Google Scholar
Mortara, R. A. Google Scholar
Rabinovitch, M. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Coxiella burnetii, the agent of Q fever in man and of coxiellosis in other species, is an intracellular pathogen not yet grown axenically. Confocal laser fluorescence microscopy and morphometry were used to measure relative C. burnetii phase II loads and their intracellular distribution in aldehyde fixed and DAPI stained Vero cell monolayers. the fluorescence of single horizontal optical sections provided useful information on relative loads of bacteria in cells and vacuoles. the relative density of the bacteria in the vacuoles was inferred from ratios of fluorescence to vacuolar section areas. Relative bacterial loads, bacterial densities and section areas of large vacuoles increased exponentially between days 2 and 4 of the infection of gamma -irradiated host cells, stabilized between days 4 and 6, and decreased thereafter. Estimated minimum doubling times were higher for the overall complement of the intracellular organisms (about 12 h) than for bacteria that were confined to larger vacuoles (about 10 h), (C) 2001 Elsevier Science B.V. All rights reserved.
Palavra-chave confocal laser fluorescence microscopy
Coxiella burnetii phase II
intracellular infection
phagosomes
parasitophorous vacuoles
quantitative fluorescence
Idioma Inglês
Data de publicação 2001-01-01
Publicado em Journal of Microbiological Methods. Amsterdam: Elsevier B.V., v. 43, n. 3, p. 223-232, 2001.
ISSN 0167-7012 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 223-232
Fonte http://dx.doi.org/10.1016/S0167-7012(00)00223-2
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000166598000007
Endereço permanente http://repositorio.unifesp.br/handle/11600/26446

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