Occurrence of heparin in the invertebrate Styela plicata (Tunicata) is restricted to cell layers facing the outside environment - An ancient role in defense?

Occurrence of heparin in the invertebrate Styela plicata (Tunicata) is restricted to cell layers facing the outside environment - An ancient role in defense?

Autor Cavalcante, MCM Google Scholar
Allodi, S. Google Scholar
Valente, A. P. Google Scholar
Straus, Anita Hilda Autor UNIFESP Google Scholar
Takahashi, Helio Kiyoshi Autor UNIFESP Google Scholar
Mourao, PAS Google Scholar
Pavao, MSG Google Scholar
Instituição Universidade Federal do Rio de Janeiro (UFRJ)
Universidade Federal de São Paulo (UNIFESP)
Resumo Heparin is an intracellular product of vertebrate mast cell currently used as exogenous anticoagulant. Despite of the potent biological activities of exogenous heparin, its physiological function has not been clearly established yet. Here, a heparin with similar structure and anticoagulant properties to the mammalian counterpart was shown to occur as the intracellular product of test cells, a cell monolayer that surrounds egg of the invertebrate Styela plicata (Chordata-Tunicata). As in the case of mammalian mast cells, heparin from the ascidian test cells is removed from the intracellular granules after incubation with compound 48/80, Following fertilization, the test cells surrounding the developing larva still retain heparin as metachromatic granulation. in the adult invertebrate, heparin occurs as intracellular granules at the apical tip of epithelial cells surrounding the lumen of both intestine and pharynx, in close contact with the external environment. This is the first description of the presence of heparin in cytoplasmic granules of epithelial-like cells around the lumen of sites exposed to external agents. This arrangement may reflect the participation of heparin in defense mechanisms in this invertebrate.
Idioma Inglês
Data 2000-11-17
Publicado em Journal of Biological Chemistry. Bethesda: Amer Soc Biochemistry Molecular Biology Inc, v. 275, n. 46, p. 36189-36196, 2000.
ISSN 0021-9258 (Sherpa/Romeo, fator de impacto)
Editor Amer Soc Biochemistry Molecular Biology Inc
Extensão 36189-36196
Fonte http://dx.doi.org/10.1074/jbc.M005830200
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000165382000077
URI http://repositorio.unifesp.br/handle/11600/26414

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