Identification of human chromosome 22 transcribed sequences with ORF expressed sequence tags

Identification of human chromosome 22 transcribed sequences with ORF expressed sequence tags

Autor Souza, Sandro José de Google Scholar
Camargo, Anamaria A. Google Scholar
Briones, Marcelo Ribeiro da Silva Autor UNIFESP Google Scholar
Costa, Fernando Ferreira Google Scholar
Nagai, Maria Aparecida Google Scholar
Verjovski-Almeida, Sergio Google Scholar
Zago, Marco Antonio Google Scholar
Andrade, Luiz Eduardo Coelho Autor UNIFESP Google Scholar
Carrer, Helaine Google Scholar
El-Dorry, Hamza FA Google Scholar
Espreafico, Enilza M. Google Scholar
Habr-Gama, Angelita Google Scholar
Giannella-Neto, Daniel Google Scholar
Goldman, Gustavo Henrique Google Scholar
Gruber, Arthur Google Scholar
Hackel, Christine Google Scholar
Kimura, Edna Teruko Autor UNIFESP Google Scholar
Maciel, Rui Monteiro de Barros Autor UNIFESP Google Scholar
Marie, Suely KN Google Scholar
Martins, Elizabeth AL Google Scholar
Nobrega, Marina P. Google Scholar
Paçó-Larson, Maria Luisa Google Scholar
Pardini, Maria Ines MC Google Scholar
Pereira, Gonçalo G. Google Scholar
Pesquero, João Bosco Autor UNIFESP Google Scholar
Rodrigues, Vanderlei Google Scholar
Rogatto, Silvia R Google Scholar
Silva, Ismael Dale Cotrim Guerreiro da Autor UNIFESP Google Scholar
Sogayar, Mari C. Google Scholar
Sonati, Maria de Fátima Google Scholar
Tajara, Eloisa H. Google Scholar
Valentini, Sandro R Google Scholar
Acencio, Marcio Google Scholar
Alberto, Fernando L. Google Scholar
Amaral, Maria Elisabete J Google Scholar
Veiga, Silvio Sanches Autor UNIFESP Google Scholar
Travassos, Luiz Rodolpho Autor UNIFESP Google Scholar
Aneas, Ivy Google Scholar
Bengtson, Mario Henrique Google Scholar
Carraro, Dirce Maria Google Scholar
Carvalho, Alex Fiorini de Google Scholar
Carvalho, Lucia Helena Google Scholar
Cerutti, Janete Maria Autor UNIFESP Google Scholar
Correa, Maria Lucia C Google Scholar
Costa, Maria Cristina R Google Scholar
Curcio, Cyntia Google Scholar
Gushiken, Tsieko Google Scholar
Ho, Paulo L. Google Scholar
Kimura, Elza Google Scholar
Leite, Luciana CC Google Scholar
Maia, Gustavo Google Scholar
Majumder, Paromita Google Scholar
Marins, Mozart Google Scholar
Matsukuma, Adriana Google Scholar
Melo, Analy Salles de Azevedo Autor UNIFESP Google Scholar
Mestriner, Carlos Alberto Google Scholar
Miracca, Elisabete C. Google Scholar
Miranda, Daniela C. Google Scholar
Nascimento, Ana Lucia Tabet Oller do Google Scholar
Nobrega, Francisco G. Google Scholar
Ojopi, Elida PB Google Scholar
Pandolfi, José Rodrigo C Google Scholar
Pessoa, Luciana Gilbert Autor UNIFESP Google Scholar
Rahal, Paula Google Scholar
Rainho, Claudia A. Google Scholar
Ro's, Nancy da Google Scholar
Sa, Renata G. de Google Scholar
Sales, Magaly M. Google Scholar
Silva, Neusa Pereira da Autor UNIFESP Google Scholar
Silva, Tereza C. Google Scholar
Silva Junior, Wilson da Google Scholar
Simao, Daniel F. Google Scholar
Sousa, Josane F. Google Scholar
Stecconi, Daniella Google Scholar
Tsukumo, Fernando Google Scholar
Valente, Valeria Google Scholar
Zalcberg, Heloisa Google Scholar
Brentani, Ricardo Renzo Autor UNIFESP Google Scholar
Reis, Luiz Fernando Lima Google Scholar
Dias-Neto, Emmanuel Google Scholar
Simpson, Andrew JG Google Scholar
Instituição Ludwig Inst Canc Res
Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Campinas (UNICAMP)
Universidade de São Paulo (USP)
Ctr Biotechnol
Univ Vale do Paraiba
Univ Estadual Paulista
Resumo Transcribed sequences in the human genome can be identified with confidence only by alignment with sequences derived from cDNAs synthesized from naturally occurring mRNAs. We constructed a set of 250,000 cDNAs that represent partial expressed gene sequences and that are biased toward the central coding regions of the resulting transcripts. They are termed ORF expressed sequence tags (ORESTES). the 250,000 ORESTEs were assembled into 81,429 contigs. of these, 1,181 (1.45%) were found to match sequences in chromosome 22 with at least one ORESTES contig for 162 (65.6%) of the 247 known genes, for 67 (44.6%) of the 150 related genes, and for 45 of the 148 (30.4%) EST-predicted genes on this chromosome. Using a set of stringent criteria to validate our sequences, we identified a further 219 previously unannotated transcribed sequences on chromosome 22. of these, 171 were in fact also defined by EST or full length cDNA sequences available in GenBank but not utilized in the initial annotation of the first human chromosome sequence. Thus despite representing less than 15% of all expressed human sequences in the public databases at the time of the present analysis, ORESTEs sequences defined 48 transcribed sequences on chromosome 22 not defined by other sequences. All of the transcribed sequences defined by ORESTEs coincided with DNA regions predicted as encoding exons by GENSCAN.
Idioma Inglês
Data de publicação 2000-11-07
Publicado em Proceedings of the National Academy of Sciences of the United States of America. Washington: Natl Acad Sciences, v. 97, n. 23, p. 12690-12693, 2000.
ISSN 0027-8424 (Sherpa/Romeo, fator de impacto)
Publicador Natl Acad Sciences
Extensão 12690-12693
Fonte http://dx.doi.org/10.1073/pnas.97.23.12690
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000165225800062
Endereço permanente http://repositorio.unifesp.br/handle/11600/26413

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