A role for activin A and betacellulin in human fetal pancreatic cell differentiation and growth

A role for activin A and betacellulin in human fetal pancreatic cell differentiation and growth

Autor Demeterco, Carla Autor UNIFESP Google Scholar
Beattie, Gillian M. Google Scholar
Dib, Sergio Atala Autor UNIFESP Google Scholar
Lopez, Ana D.] Google Scholar
Hayek, Alfredo Google Scholar
Instituição Univ Calif San Diego
Universidade Federal de São Paulo (UNIFESP)
Resumo Activin A (Act.A), a member of the transforming growth factor beta family of secreted proteins, has been implicated in the regulation of growth and differentiation of various cell types. Betacellulin (BTC), a member of the epidermal growth factor family, converts exocrine AR42J cells to insulin-expressing cells when combined with Act.A. We have used primary cultures of human fetal pancreatic tissue to identify the effects of Act.A and/or ETC on islet development and growth. Exposure to Act.A resulted in a 1.5-fold increase in insulin content (P < 0.005) and a 2-fold increase in the number of cells immunopositive for insulin (P < 0.005). the formation of islet-like cell clusters, containing mainly epithelial cells, during a Ei-day culture, was stimulated 1.4-fold by ETC (P < 0.05). ETC alone caused a 2.6-fold increase in DNA synthesis (P < 0.005). These data suggest that Act.A induces endocrine differentiation, whereas BTC has a mitogenic effect on human undifferentiated pancreatic epithelial cells.
Idioma Inglês
Data de publicação 2000-10-01
Publicado em Journal of Clinical Endocrinology & Metabolism. Chevy Chase: Endocrine Soc, v. 85, n. 10, p. 3892-3897, 2000.
ISSN 0021-972X (Sherpa/Romeo, fator de impacto)
Publicador Endocrine Soc
Extensão 3892-3897
Fonte http://dx.doi.org/10.1210/jcem.85.10.6848
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000089820800068
Endereço permanente http://repositorio.unifesp.br/handle/11600/26390

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