Prevalence of HEp-2 cell-adherent Escherichia coli and characterisation of enteroaggregative E-coli and chain-like adherent E-coli isolated from children with and without diarrhoea, in Londrina, Brazil

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dc.contributor.author Gioppo, Nereida MR
dc.contributor.author Elias Junior, Waldir Pereira [UNIFESP]
dc.contributor.author Vidotto, Marilda C.
dc.contributor.author Linhares, Rosa E.
dc.contributor.author Saridakis, Halha O.
dc.contributor.author Gomes, Tania Aparecida Tardelli [UNIFESP]
dc.contributor.author Trabulsi, Luiz R.
dc.contributor.author Pelayo, Jacinta S.
dc.date.accessioned 2016-01-24T12:31:10Z
dc.date.available 2016-01-24T12:31:10Z
dc.date.issued 2000-09-15
dc.identifier http://dx.doi.org/10.1111/j.1574-6968.2000.tb09301.x
dc.identifier.citation Fems Microbiology Letters. Amsterdam: Elsevier B.V., v. 190, n. 2, p. 293-298, 2000.
dc.identifier.issn 0378-1097
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/26384
dc.description.abstract A total of 919 Escherichia coli isolates from 125 children with diarrhoea (cases) and 98 controls were assayed for adherence to HEp-2 cells. Localised adherence was found only in isolates from cases. Diffuse, aggregative (AA), chain-like adherence (CLA) and variants of the AA pattern were found in both cases and controls. the AA isolates were tested for gene sequences associated with enteroaggregative E. coli (EAEC). Only 25% of the isolates hybridised with the EAEC probe, and the aafA, astA and pet gene sequences were found in 7.9%, 44.7% and 7.9% of the isolates, respectively. the aggA gene was not found, although 7.9% were positive for aggC. the CLA isolates reacted with the EAEC probe (55.6%),nd the aggC, astA and pet gene sequences were found in 66.7%, 33.3% and 11.1%, respectively. the aggR (55.6%), aspU (55.6%), shf(33.3%) and she (22.2%) genes were also found in CLA isolates. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. en
dc.format.extent 293-298
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Fems Microbiology Letters
dc.rights Acesso aberto
dc.subject adherence pattern en
dc.subject enteroaggregative Escherichia coli en
dc.subject chain-like adhesion en
dc.subject virulence marker en
dc.title Prevalence of HEp-2 cell-adherent Escherichia coli and characterisation of enteroaggregative E-coli and chain-like adherent E-coli isolated from children with and without diarrhoea, in Londrina, Brazil en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Estadual de Londrina (UEL)
dc.contributor.institution Inst Butantan
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ Estadual Londrina, Dept Microbiol, BR-86051970 Londrina, Pr, Brazil
dc.description.affiliation Inst Butantan, Lab Especial Microbiol, BR-05503990 São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.identifier.doi 10.1111/j.1574-6968.2000.tb09301.x
dc.description.source Web of Science
dc.identifier.wos WOS:000089209700019



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