A heme-binding aspartic proteinase from the eggs of the hard tick Boophilus microplus

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dc.contributor.author Sorgine, MHF
dc.contributor.author Logullo, C.
dc.contributor.author Zingali, R. B.
dc.contributor.author Paiva-Silva, G. O.
dc.contributor.author Juliano, Luiz [UNIFESP]
dc.contributor.author Oliveira, P. L.
dc.date.accessioned 2016-01-24T12:31:10Z
dc.date.available 2016-01-24T12:31:10Z
dc.date.issued 2000-09-15
dc.identifier http://dx.doi.org/10.1074/jbc.M005675200
dc.identifier.citation Journal of Biological Chemistry. Bethesda: Amer Soc Biochemistry Molecular Biology Inc, v. 275, n. 37, p. 28659-28665, 2000.
dc.identifier.issn 0021-9258
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/26383
dc.description.abstract An aspartic proteinase that binds heme with a 1:1 stoichiometry was isolated and cloned from the eggs of the cattle tick Boophilus microplus. This proteinase, herein named THAP (tick heme-binding aspartic proteinase) showed pepstatin-sensitive hydrolytic activity against several peptide and protein substrates, Although hemoglobin was a good substrate for THAP, low proteolytic activity was observed against globin devoid of the heme prosthetic group. Hydrolysis of globin by THAP increased as increasing amounts of heme were added to globin, with maximum activation at a heme-to-globin 1:1 ratio. Further additions of heme to the reaction medium inhibited proteolysis, back to a level similar to that observed against globin alone. the addition of heme did not change THAP activity toward a synthetic peptide or against ribonuclease, a non-hemeprotein substrate. the major storage protein of tick eggs, vitellin (VT), the probable physiological substrate of THAP, is a hemeprotein. Hydrolysis of VT by THAP was also inhibited by the addition of heme to the incubation media. Taken together, our results suggest that THAP uses heme bound to VT as a docking site to increase specificity and regulate VT degradation according to heme availability. en
dc.format.extent 28659-28665
dc.language.iso eng
dc.publisher Amer Soc Biochemistry Molecular Biology Inc
dc.relation.ispartof Journal of Biological Chemistry
dc.rights Acesso aberto
dc.title A heme-binding aspartic proteinase from the eggs of the hard tick Boophilus microplus en
dc.type Artigo
dc.contributor.institution Universidade Federal do Rio de Janeiro (UFRJ)
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Universidade Federal Fluminense (UFF)
dc.description.affiliation Univ Fed Rio de Janeiro, Ctr Ciencias Saude, Inst Ciencias Biomed, Dept Bioquim Med, BR-21910590 Rio de Janeiro, Brazil
dc.description.affiliation UNIFESP, Escola Paulista Med, Dept Biofis, BR-04044020 São Paulo, Brazil
dc.description.affiliation Univ Fed Fluminense, Inst Biol, Dept Biol Celular & Mol, BR-24001970 Niteroi, RJ, Brazil
dc.description.affiliationUnifesp UNIFESP, Escola Paulista Med, Dept Biofis, BR-04044020 São Paulo, Brazil
dc.identifier.doi 10.1074/jbc.M005675200
dc.description.source Web of Science
dc.identifier.wos WOS:000089330700044


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