Simultaneous registration of contraction and cytosolic calcium ([Ca2+](i)) of smooth muscle strips using front-surface fluorimetry

Simultaneous registration of contraction and cytosolic calcium ([Ca2+](i)) of smooth muscle strips using front-surface fluorimetry

Autor Ferreira, A. T. Google Scholar
Neri, R. Google Scholar
Oshiro, MEM Google Scholar
Kanaide, H. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Kyushu Univ
Resumo Using front-surface fluorimetry with fura-2-loaded smooth muscle strips, simultaneous registration of the cytosolic calcium concentration ([Ca2+](i)) changes and tension development was done under the action of 40 mM KCl and the myotropic peptide 10(-6) M angiotensin II. the strips were mounted vertically, connected to a force transducer that keeps a basal isometric tension of 0.5 g, and maintained in a bathing solution oxygenated at 37 degrees C. the fiber-optic platform FluoroMax-2 accessory 1950F was used to do the remote sensing for the samples. Light from the excitation spectrometer (FluoroMax-2), alternating between 340 and 380 nm, was focused onto the fiber-optic bundle and directed to the smooth muscle strip. the fluorescence (505 nm) was collected and redirected to the emission port of the fluorimeter FluoroMax-2. the ratiometric method (R340/380) was used as an index of [Ca2+](i) change during smooth muscle contraction. All data, R340/380 and tension, were recorded using a computerized data acquisition system: Soft & Solution and GRAMS/386 of Galatic Industries Corporation.
Palavra-chave fura-2
front surface
smooth muscle
cytosolic calcium
Idioma Inglês
Data de publicação 2000-09-01
Publicado em Journal of Fluorescence. New York: Plenum Publ Corp, v. 10, n. 3, p. 223-228, 2000.
ISSN 1053-0509 (Sherpa/Romeo, fator de impacto)
Publicador Plenum Publ Corp
Extensão 223-228
Fonte http://dx.doi.org/10.1023/A:1009468203995
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000088419500002
Endereço permanente http://repositorio.unifesp.br/handle/11600/26377

Exibir registro completo




Arquivo

Arquivo Tamanho Formato Visualização

Não existem arquivos associados a este item.

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta