Highly-substituted benzhydrylamine resin: An alternative chromatographic support for ganglioside and neutral glycosphingolipid purification

Highly-substituted benzhydrylamine resin: An alternative chromatographic support for ganglioside and neutral glycosphingolipid purification

Autor Carvalho, RSH Google Scholar
Straus, A. H. Google Scholar
Takahashi, H. K. Google Scholar
Nakaie, C. R. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Benzhydrylamine resin (BHAR) is a copolymer of (styrene-1% divinylbenzene) containing phenylmethylamine groups and commonly used as a solid support for peptide synthesis in organic solvents. We have demonstrated that a larger number of NH3+ groups distributed throughout the BHAR matrix improves bead solvation under more polar conditions. As this characteristic might allow this aminated-polymer to be used as a stationary phase for liquid chromatography, a highly substituted BHAR (2.4 mmol.g(-1) of amine groups) was synthesized under forceful conditions. Neutral glycosphingolipids and negatively charged gangliosides and sulfatide obtained from rat brain extracts were successfully purified and fractionated in this BHAR batch by single-step, reverse-phase anion-exchange chromatography. Additionally, the anion-exchange potential of BHAR was also compared to that of commercial dimethylaminoethyl (DEAE)-Sephadex A25 resin.
Palavra-chave thin-layer chromatography
column liquid chromatography
benzhydrylamine resin
gangliosides
glycosphingolipids
peptides
Idioma Inglês
Data de publicação 2000-09-01
Publicado em Chromatographia. Wiesbaden: Vieweg, v. 52, n. 5-6, p. 363-366, 2000.
ISSN 0009-5893 (Sherpa/Romeo, fator de impacto)
Publicador Vieweg
Extensão 363-366
Fonte http://dx.doi.org/10.1007/BF02491033
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000089660600018
Endereço permanente http://repositorio.unifesp.br/handle/11600/26368

Exibir registro completo




Arquivo

Arquivo Tamanho Formato Visualização

Não existem arquivos associados a este item.

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta