Cleaning of blood-contaminated reprocessed angiographic catheters and spinal needles

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dc.contributor.author Penna, TCV
dc.contributor.author Ferraz, CAM
dc.date.accessioned 2016-01-24T12:31:08Z
dc.date.available 2016-01-24T12:31:08Z
dc.date.issued 2000-08-01
dc.identifier http://dx.doi.org/10.1086/501793
dc.identifier.citation Infection Control and Hospital Epidemiology. Thorofare: Slack Inc, v. 21, n. 8, p. 499-504, 2000.
dc.identifier.issn 0899-823X
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/26356
dc.description.abstract OBJECTIVES: To evaluate the efficacy of a multistep cleaning method using a cleaner and a chemical disinfectant on blood-contaminated angiographic catheters and spinal needles intended to be sterilized by hydrogen peroxide gas plasma.METHOD: A mixture of radiopaque iodine contrast, bovine blood (plus ethylenediaminetetraacetic acid), and a suspension of Bacillus subtilis spores was used to simulate catheterization and needle use. the mixture was a 1:1 proportion of con trast and blood, inoculated so that there was a final concentration of B subtilis spores of 1.0 x 10(6) colony-forming units (CFU)/mL. the inoculated devices were cleaned using a hydrogen peroxide solution at a concentration of 1.5 +/- 0.5 percent by weight, followed by distilled water with enzymatic detergent. After drying, the devices were sterilized with hydrogen peroxide gas plasma.RESULTS: the initial B subtilis spore concentration inoculated into catheters and needles varied from 2.12 x 10(4) to 2.74 x 10(7) CFU/mL. the residual load of B subtilis spores after cleaning varied from zero (no count) to a maximum of 200 CFU/device. the multistep cleaning procedure was responsible for an average 5-log(10) reduction of B subtilis spores in the catheter and needle lumens.CONCLUSIONS: the hydrogen peroxide and enzymatic detergent aqueous solutions were shown to be efficacious when used as part of a multistep cleaning method. the low level of microbial contamination prior to sterilization with hydrogen peroxide gas plasma assured that the intended sterility assurance level was reached (Infect Control Hosp Epidemiol 2000;21:499-504). en
dc.format.extent 499-504
dc.language.iso eng
dc.publisher Slack Inc
dc.relation.ispartof Infection Control and Hospital Epidemiology
dc.rights Acesso restrito
dc.title Cleaning of blood-contaminated reprocessed angiographic catheters and spinal needles en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Johnson & Johnson Prod Profiss LTDA Rodovia Presi
dc.description.affiliation Universidade Federal de São Paulo, Sch Pharmaceut Sci, Dept Pharmaceut Technol & Biochem, São Paulo, Brazil
dc.description.affiliation Johnson & Johnson Prod Profiss LTDA Rodovia Presi, Sao Jose Dos Campos, SP, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Sch Pharmaceut Sci, Dept Pharmaceut Technol & Biochem, São Paulo, Brazil
dc.identifier.doi 10.1086/501793
dc.description.source Web of Science
dc.identifier.wos WOS:000088795200005



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