Cleaning of blood-contaminated reprocessed angiographic catheters and spinal needles

Cleaning of blood-contaminated reprocessed angiographic catheters and spinal needles

Author Penna, TCV Google Scholar
Ferraz, CAM Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Johnson & Johnson Prod Profiss LTDA Rodovia Presi
Abstract OBJECTIVES: To evaluate the efficacy of a multistep cleaning method using a cleaner and a chemical disinfectant on blood-contaminated angiographic catheters and spinal needles intended to be sterilized by hydrogen peroxide gas plasma.METHOD: A mixture of radiopaque iodine contrast, bovine blood (plus ethylenediaminetetraacetic acid), and a suspension of Bacillus subtilis spores was used to simulate catheterization and needle use. the mixture was a 1:1 proportion of con trast and blood, inoculated so that there was a final concentration of B subtilis spores of 1.0 x 10(6) colony-forming units (CFU)/mL. the inoculated devices were cleaned using a hydrogen peroxide solution at a concentration of 1.5 +/- 0.5 percent by weight, followed by distilled water with enzymatic detergent. After drying, the devices were sterilized with hydrogen peroxide gas plasma.RESULTS: the initial B subtilis spore concentration inoculated into catheters and needles varied from 2.12 x 10(4) to 2.74 x 10(7) CFU/mL. the residual load of B subtilis spores after cleaning varied from zero (no count) to a maximum of 200 CFU/device. the multistep cleaning procedure was responsible for an average 5-log(10) reduction of B subtilis spores in the catheter and needle lumens.CONCLUSIONS: the hydrogen peroxide and enzymatic detergent aqueous solutions were shown to be efficacious when used as part of a multistep cleaning method. the low level of microbial contamination prior to sterilization with hydrogen peroxide gas plasma assured that the intended sterility assurance level was reached (Infect Control Hosp Epidemiol 2000;21:499-504).
Language English
Date 2000-08-01
Published in Infection Control and Hospital Epidemiology. Thorofare: Slack Inc, v. 21, n. 8, p. 499-504, 2000.
ISSN 0899-823X (Sherpa/Romeo, impact factor)
Publisher Slack Inc
Extent 499-504
Origin http://dx.doi.org/10.1086/501793
Access rights Closed access
Type Article
Web of Science ID WOS:000088795200005
URI http://repositorio.unifesp.br/handle/11600/26356

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