Cleaning of blood-contaminated reprocessed angiographic catheters and spinal needles

Cleaning of blood-contaminated reprocessed angiographic catheters and spinal needles

Autor Penna, TCV Google Scholar
Ferraz, CAM Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Johnson & Johnson Prod Profiss LTDA Rodovia Presi
Resumo OBJECTIVES: To evaluate the efficacy of a multistep cleaning method using a cleaner and a chemical disinfectant on blood-contaminated angiographic catheters and spinal needles intended to be sterilized by hydrogen peroxide gas plasma.METHOD: A mixture of radiopaque iodine contrast, bovine blood (plus ethylenediaminetetraacetic acid), and a suspension of Bacillus subtilis spores was used to simulate catheterization and needle use. the mixture was a 1:1 proportion of con trast and blood, inoculated so that there was a final concentration of B subtilis spores of 1.0 x 10(6) colony-forming units (CFU)/mL. the inoculated devices were cleaned using a hydrogen peroxide solution at a concentration of 1.5 +/- 0.5 percent by weight, followed by distilled water with enzymatic detergent. After drying, the devices were sterilized with hydrogen peroxide gas plasma.RESULTS: the initial B subtilis spore concentration inoculated into catheters and needles varied from 2.12 x 10(4) to 2.74 x 10(7) CFU/mL. the residual load of B subtilis spores after cleaning varied from zero (no count) to a maximum of 200 CFU/device. the multistep cleaning procedure was responsible for an average 5-log(10) reduction of B subtilis spores in the catheter and needle lumens.CONCLUSIONS: the hydrogen peroxide and enzymatic detergent aqueous solutions were shown to be efficacious when used as part of a multistep cleaning method. the low level of microbial contamination prior to sterilization with hydrogen peroxide gas plasma assured that the intended sterility assurance level was reached (Infect Control Hosp Epidemiol 2000;21:499-504).
Idioma Inglês
Data de publicação 2000-08-01
Publicado em Infection Control and Hospital Epidemiology. Thorofare: Slack Inc, v. 21, n. 8, p. 499-504, 2000.
ISSN 0899-823X (Sherpa/Romeo, fator de impacto)
Publicador Slack Inc
Extensão 499-504
Fonte http://dx.doi.org/10.1086/501793
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000088795200005
Endereço permanente http://repositorio.unifesp.br/handle/11600/26356

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