Spectrophotometric determination of total nitrogen in plant materials using a flow-injection system with an AgCl(s) reactor

Spectrophotometric determination of total nitrogen in plant materials using a flow-injection system with an AgCl(s) reactor

Autor Carneiro, JMT Google Scholar
Sartini, R. P. Google Scholar
Zagatto, EAG Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo An improved flow-injection system with an AgCl(s) reactor is proposed for the spectrophotometric determination of total nitrogen in Kjeldahl digests of plant materials. After sample injection, the established sample plug is alkalinised and allowed to flow through the mini-column where Ag+ ions are displaced as the soluble [Ag(NH3)(2)](+) complex. Bromopyrogallol red and o-phenanthroline are then added and a coloured ternary complex is formed under neutral conditions. the system is very stable, and baseline drift is usually <0.01 absorbance after 4 h operation periods. Precise results (R.S.D.<2%) are obtained at a rate of 100 h(-1). the analytical curve is linear up to 6.00% (w/w) N (dry basis), regression coefficient has been estimated as >0.999 (n=6) and detection limit is 0.2% (w/w) N. Accuracy was confirmed by running standard reference materials and typical samples already assayed by conductimetry. (C) 2000 Elsevier Science B.V. All rights reserved.
Palavra-chave flow analysis
total nitrogen
plant material
spectrophotometry
mini-column
silver chloride reactor
Idioma Inglês
Data de publicação 2000-07-10
Publicado em Analytica Chimica Acta. Amsterdam: Elsevier B.V., v. 416, n. 2, p. 185-190, 2000.
ISSN 0003-2670 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 185-190
Fonte http://dx.doi.org/10.1016/S0003-2670(00)00898-9
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000087961900008
Endereço permanente http://repositorio.unifesp.br/handle/11600/26345

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