CD4 Th1 but not Th2 clones efficiently activate macrophages to eliminate Trypanosoma cruzi through a nitric oxide dependent mechanism

CD4 Th1 but not Th2 clones efficiently activate macrophages to eliminate Trypanosoma cruzi through a nitric oxide dependent mechanism

Autor Rodrigues, M. M. Google Scholar
Ribeirao, M. Google Scholar
Boscardin, S. B. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo We have recently generated CD4 clones from BALB/c mice immunized with a plasmid DNA containing the gene encoding for the catalytic domain of trans-sialidase, an important enzyme expressed on the surface of Trypanosoma cruzi trypomastigotes. These clones allowed us to study in vitro the interaction between T cells and T. cruzi-infected macrophages. A cytotoxic CD4 clone of the Th1 type effectively activated macrophages to kill intracellular amastigote forms of T. cruzi. in contrast, CD4 Th2-like clones were much less efficient, being unable to activate macrophages to significantly reduce parasite development. We found that the anti-parasitic activity of Th1 cells was completely suppressed by the presence of nitric oxide synthase inhibitors. Also, we observed that anti-IFN-gamma antibodies significantly inhibited the anti-parasitic activity of these cells. We conclude that trypomastigote-specific Th1 cells activate macrophages to kill intracellular amastigotes of T. cruzi by a mechanism exclusively dependent on the induction of nitric oxide synthesis. (C) 2000 Elsevier Science B.V. All rights reserved.
Palavra-chave Trypanosoma cruzi
CD4 Th1 clone
nitric oxide
Idioma Inglês
Data de publicação 2000-07-03
Publicado em Immunology Letters. Amsterdam: Elsevier B.V., v. 73, n. 1, p. 43-50, 2000.
ISSN 0165-2478 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 43-50
Fonte http://dx.doi.org/10.1016/S0165-2478(00)00205-4
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000088604900008
Endereço permanente http://repositorio.unifesp.br/handle/11600/26343

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