DNA-based vaccination against murine paracoccidioidomycosis using the gp43 gene from Paracoccidioides brasiliensis

DNA-based vaccination against murine paracoccidioidomycosis using the gp43 gene from Paracoccidioides brasiliensis

Autor Pinto, A. R. Google Scholar
Puccia, R. Google Scholar
Diniz, S. N. Google Scholar
Franco, M. F. Google Scholar
Travassos, L. R. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Inst Adolfo Lutz
Universidade Federal de Minas Gerais (UFMG)
Resumo Gp43, the major 43-kDa antigenic glycoprotein of Paracoccidioides brasiliensis, or its 15-amino acid inner peptide (P10), induces a T-CD4(+), Th1 cellular immune response which protects BALB/c mice from intratracheal infection by virulent yeast forms. We investigated whether DNA vaccination using the gp43 gene could elicit protective immunity against P. brasiliensis. Animals immunised intramuscularly (i.m.) or intradermally (i.d.) with plasmid DNA containing the gp43 gene induced a specific, long lasting humoral and cellular immune response. A mixed Th1/Th2 cellular immune response in DNA-immunized mice was modulated in vivo by IFN-gamma and was protective in BALB/c mice. A significant decrease in the lung colony forming units (CFUs) and reduced, or no dissemination to the spleen and liver of immunised mice were observed. (C) 2000 Elsevier B.V. All rights reserved.
Palavra-chave Paracoccidioines brasiliensis
DNA immunisation
gp43
Idioma Inglês
Data de publicação 2000-07-01
Publicado em Vaccine. Oxford: Elsevier B.V., v. 18, n. 26, p. 3050-3058, 2000.
ISSN 0264-410X (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 3050-3058
Fonte http://dx.doi.org/10.1016/S0264-410X(00)00074-8
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000087890000018
Endereço permanente http://repositorio.unifesp.br/handle/11600/26336

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