The liver monooxygenase system of Brazilian freshwater fish

The liver monooxygenase system of Brazilian freshwater fish

Autor Leitao, MAS Google Scholar
Affonso, E. G. Google Scholar
Silva, MFE da Google Scholar
Meirelles, N. C. Google Scholar
Rantin, F. T. Google Scholar
Vercesi, A. E. Google Scholar
Junqueira, VCB Google Scholar
Degterev, I. A. Google Scholar
Instituição Universidade Federal de São Carlos (UFSCar)
Universidade de São Paulo (USP)
Universidade Estadual de Campinas (UNICAMP)
Universidade Federal de São Paulo (UNIFESP)
Russian Acad Sci
Resumo Content of cytochromes b(5) and P-450, and activities of NADPH-cytochrome c (P-450) reductase (NCR) and 7-ethoxyresorufin O-deethylase (EROD) were measured in liver microsomes prepared from two South American endemic fish, Brycon cephalus and Colossoma macropomum, from tilapia, Oreochromis niloticus, and from Swiss mice, Mus musculus. which served as a control. Strong hemoglobin binding to fish liver microsomal membranes (FLM) altered visible spectra of microsomal cytochromes. Consequently, special precautions during FLM preparation, including liver perfusion followed by repeated washing of microsomes, were required in the study of microsomal cytochromes from these fish. FLM from all fish studied here had a significantly lower content of microsomal cytochromes but a similar level of NCR and EROD activities compared to mouse liver microsomes (MLM). Strong response of the monooxygenase system in O. niloticus to water pollution was detected with both specific cytochrome P-450 content and EROD activity increasing sharply. the optical spectra of hemoglobin from B. cephalus and C. macropomum were analyzed and some differences in shape and relative extinction were observed compared to known hemoglobins. (C) 2000 Elsevier Science Inc. All lights reserved.
Palavra-chave cytochrome P-450
cytochrome b(5)
hemoglobin
liver microsomes
neotropical fish
EROD
NCR activity
Idioma Inglês
Data de publicação 2000-05-01
Publicado em Comparative Biochemistry and Physiology C-pharmacology Toxicology & Endocrinology. Oxford: Pergamon-Elsevier B.V., v. 126, n. 1, p. 29-38, 2000.
ISSN 1367-8280 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 29-38
Fonte http://dx.doi.org/10.1016/S0742-8413(00)00092-X
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000087752100004
Endereço permanente http://repositorio.unifesp.br/handle/11600/26305

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