Effect of epithelial debridement on glycosaminoglycan synthesis by human corneal explants

Effect of epithelial debridement on glycosaminoglycan synthesis by human corneal explants

Autor Soriano, E. S. Google Scholar
Campos, MSQ Google Scholar
Michelacci, Y. M. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo The purpose of the present work was to investigate the effects of mechanical epithelial debridement upon glycosaminoglycan synthesis by human corneal explants Corneal explants were maintained under tissue culture conditions for 2-72 days and the glycosaminoglycans synthesized in 24 h were metabolically labeled by addition of S-35-sulfate to the culture medium. These compounds were isolated from the tissue explants and analyzed by a combination of agarose gel electrophoresis and enzymatic degradation with specific mucopolysaccharidases. the glycosaminoglycans synthesized by isolated epithelial cells and by corneas previously submitted to epithelial cell debridement were compared to controls. Keratan sulfate (26 kDa) and dermatan sulfate (43 kDa) were the main corneal glycosaminoglycans,each one corresponding to about 50% of the total. Nevertheless, the main S-35-labeled glycosaminoglycan was S-35-dermatan sulfate (73%), with smaller amounts of S-35-keratan sulfate (15%) and S-35-heparan sulfate (12%), suggesting a lower synthesis rate for keratan sulfate. the main glycosaminoglycan synthesized by isolated epithelial cells was heparan sulfate. the removal of epithelial layer caused a decrease in heparan sulfate labeling and induced the synthesis of dermatan sulfate by stromal cells. This increased synthesis of dermatan sulfate suggest a relationship between epithelium and stroma and could be related to the corneal opacity that may appear after epithelial cell debridement. (C) 2000 Elsevier Science B.V. All rights reserved.
Palavra-chave corneal explants
epithelial debridement
glycosaminoglycan
Idioma Inglês
Data de publicação 2000-05-01
Publicado em Clinica Chimica Acta. Amsterdam: Elsevier B.V., v. 295, n. 1-2, p. 41-62, 2000.
ISSN 0009-8981 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 41-62
Fonte http://dx.doi.org/10.1016/S0009-8981(00)00188-1
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000086863300004
Endereço permanente http://repositorio.unifesp.br/handle/11600/26301

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