Synthesis of elastic microfibrillar components fibrillin-1 and fibrillin-2 by human optic nerve head astrocytes in situ and in vitro

Synthesis of elastic microfibrillar components fibrillin-1 and fibrillin-2 by human optic nerve head astrocytes in situ and in vitro

Autor Pena, JDO Google Scholar
Mello, PAA Google Scholar
Hernandez, M. R. Google Scholar
Instituição Washington Univ
Universidade Federal de São Paulo (UNIFESP)
Resumo The purpose of this study was to identify elastic microfibrillar components fibrillin-1 and fibrillin-2 in optic nerve heads of adult normal and glaucomatous subjects. in cultured optic nerve head astrocytes (type 1B astrocytes), as well as fibrillin-1 in fetal optic nerve heads. To characterize synthesis and gene expression of microfibrillar proteins in human optic nerve heads and cultured type 1B astrocytes, light microscopy immunohistochemistry, in situ hybridization, and RT-PCR or Northern blots were performed. Our results demonstrated that fibrillin-1 was associated with blood vessels, astrocytes in the glial columns and cribriform plates, and with astrocyte processes in the nerve bundles in all samples. in glaucomatous optic nerves there was enhanced fibrillin-1 immunoreactivity, especially surrounding blood Vessels. Fibrillin-2 was localized primarily to brood Vessels in all samples, without qualitative differences between normal and glaucomatous samples, in fetal optic nerve heads fibrillin-1. mRNA was localized to glial cells and to the blood vessel walls, in adult optic nerve heads, there was little fibrillin-1 mRNA as detectable by in situ hybridization and RT-PCR. There was no detectable upregulation of fibrillin-1 mRNA in glaucoma. in cultured type 1B astrocytes, fibrillin-1 staining was mostly pericellular.. There was little fibrillin-2 immunoreactivity. in conclusion astrocytes from the optic nerve head deposit elastic microfibrillar components in situ and in vitro, with a predominance of fibrillin-1. Upregulation of fibrillin-1 mRNA was not observed in glaucoma, suggesting that increased transcription may occur early in the disease process. Cultures of type 1B astrocytes from the optic nerve head provides a useful model to study mechanisms regulating the interactions of elastin and the microfibrils in optic nerve head astrocytes. (C) 2000 Academic Press.
Palavra-chave astrocytes
elastic fibers
fibrillin
glaucoma
optic nerve head
Idioma Inglês
Data de publicação 2000-05-01
Publicado em Experimental Eye Research. London: Academic Press Ltd, v. 70, n. 5, p. 589-601, 2000.
ISSN 0014-4835 (Sherpa/Romeo, fator de impacto)
Publicador Academic Press Ltd
Extensão 589-601
Fonte http://dx.doi.org/10.1006/exer.1999.0817
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000087321200006
Endereço permanente http://repositorio.unifesp.br/handle/11600/26300

Exibir registro completo




Arquivo

Arquivo Tamanho Formato Visualização

Não existem arquivos associados a este item.

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta