Genetic characterization of adenovirus strains isolated from patients with acute conjunctivitis in the city of São Paulo, Brazil

Genetic characterization of adenovirus strains isolated from patients with acute conjunctivitis in the city of São Paulo, Brazil

Autor Tanaka, K. Google Scholar
Itoh, N. Google Scholar
Saitoh-Inagawa, W. Google Scholar
Uchio, E. Google Scholar
Takeuchi, S. Google Scholar
Aoki, K. Google Scholar
Soriano, E. Google Scholar
Nishi, M. Google Scholar
Junior, R. B. Google Scholar
Harsi, C. M. Google Scholar
Tsuzuki-Wang, L. Google Scholar
Durigon, Edison Luiz Google Scholar
Stewien, Klaus Eberhard Google Scholar
Ohno, S. Google Scholar
Instituição Yokohama City Univ
Aoki Eye Clin
Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Resumo Genome analysis was carried out on adenovirus strains isolated from patients with acute follicular conjunctivitis in the city of São Paulo, Brazil. Eighteen conjunctival scrapings, collected between December 1993 and March 1994, were analyzed by two methods: a combination of polymerase chain reaction with restriction fragment length polymorphism and viral DNA restriction analysis, carried out using 10 restriction endonucleases: BamHI, BglI, BglII, HindIII, KpnI, SacI, SalI, SmaI, XbaI, and XhoI. Among 11 adenovirus detected by cell culture isolation, nine were Ad8, and two were Ad7. By restriction analysis the Ad8 isolates were typed as two new variants-Ad8/D11 (seven of nine samples) and Ad8/D12 (two of nine samples). Ad7 isolates were identified as a subtype of the widespread genome type Ad7b and the virulent type Ad7h, a predominant genome type circulating in Argentina, Chile, and Uruguay but absent in Brazil until 1991. J. Med. Virol 61:143-149, 2000.
Palavra-chave Brazil
acute conjunctivitis
adenovirus
new genome types
polymerase chain reaction with restriction fragment length polymorphism
genome typing
Idioma Inglês
Data de publicação 2000-05-01
Publicado em Journal of Medical Virology. New York: Wiley-liss, v. 61, n. 1, p. 143-149, 2000.
ISSN 0146-6615 (Sherpa/Romeo, fator de impacto)
Publicador Wiley-Blackwell
Extensão 143-149
Fonte http://dx.doi.org/10.1002/(SICI)1096-9071(200005)61:1<143
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000086206900023
Endereço permanente http://repositorio.unifesp.br/handle/11600/26299

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