Identification of sialic acids on the cell surface of Candida albicans

Mostrar registro simples Soares, RMA Soares, RMD Alviano, D. S. Angluster, J. Alviano, C. S. Travassos, L. R. 2016-01-24T12:31:03Z 2016-01-24T12:31:03Z 2000-04-06
dc.identifier.citation Biochimica Et Biophysica Acta-general Subjects. Amsterdam: Elsevier B.V., v. 1474, n. 2, p. 262-268, 2000.
dc.identifier.issn 0304-4165
dc.description.abstract The cell-surface expression of sialic acids in two isolates of Candida albicans was analyzed by thin-layer and gas chromatography, binding of lectins, colorimetry, sialidase treatment and flow cytofluorimetry with fluorescein-labeled lectins. N-acetylneuraminic acid (NANA) was the only derivative found in both strains of C. albicans grown in a chemically defined medium. Its identification was confirmed by mass spectrometry in comparison with an authentic standard. the density of sialic acid residues per cell ranged from 1.6x10(6) to 2.8x10(6). the surface distribution of sialic acids over the entire C. albicans was inferred from labeling with fluorescein-Limulus polyphemus and Limax flavus agglutinins and directly observed by optical microscopy with (FITC)-Sambucus nigra agglutinin (SNA), abrogated by previous treatment of yeasts with bacterial sialidase. Sialidase-treated yeasts generated beta-galactopyranosyl terminal residues that reacted with peanut agglutinin. in C. albicans N-acetyl-neuraminic acids are alpha 2,6- and alpha 2,3-linked as indicated by yeast binding to SNA and Maackia amurensis agglutinin. the alpha 2,6-linkage clearly predominated in both strains. We also investigated the contribution of sialic acids to the electronegativity of C. albicans, an important factor determining fungal interactions in vivo. Adhesion of yeast cells to a cationic solid phase substrate (poly-L-lysine) was mediated in part by sialic acids, since the number of adherent cells was significantly reduced after treatment with bacterial sialidase. the present evidence adds C. albicans to the list of pathogenic Fungi that synthesize sialic acids, which contribute to the negative charge of fungal cells and have a role in their specific interaction with the host tissue. (C) 2000 Elsevier Science B.V. All rights reserved. en
dc.format.extent 262-268
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Biochimica Et Biophysica Acta-general Subjects
dc.rights Acesso restrito
dc.subject Candida albicans en
dc.subject sialic acid en
dc.subject lectin en
dc.subject sialidase en
dc.title Identification of sialic acids on the cell surface of Candida albicans en
dc.type Artigo
dc.contributor.institution Universidade Federal do Rio de Janeiro (UFRJ)
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ Fed Rio de Janeiro, Inst Microbiol Prof Paulo Goes, Dept Microbiol Geral, BR-21941590 Rio de Janeiro, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Disciplina Biol Celular, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Disciplina Biol Celular, BR-04023062 São Paulo, Brazil
dc.identifier.doi 10.1016/S0304-4165(00)00003-9
dc.description.source Web of Science
dc.identifier.wos WOS:000086303100018

Arquivos deste item

Arquivos Tamanho Formato Visualização

Não existem arquivos associados a este item.

Este item aparece na(s) seguinte(s) coleção(s)

Mostrar registro simples