Highly purified glycosylphosphatidylinositols from Trypanosoma cruzi are potent proinflammatory agents

Highly purified glycosylphosphatidylinositols from Trypanosoma cruzi are potent proinflammatory agents

Autor Almeida, I. C. Google Scholar
Camargo, M. M. Google Scholar
Procopio, D. O. Google Scholar
Silva, Luiz S. Autor UNIFESP Google Scholar
Mehlert, A. Google Scholar
Travassos, Luiz Rodolpho Autor UNIFESP Google Scholar
Gazzinelli, R. T. Google Scholar
Ferguson, MAJ Google Scholar
Instituição Univ Dundee
Universidade Federal de Minas Gerais (UFMG)
Universidade Federal de São Paulo (UNIFESP)
Fundacao Oswaldo Cruz
Resumo Intracellular protozoan parasites are potent stimulators of cell-mediated immunity. the induction of macrophage proinflammatory cytokines by Trypanosoma cruzi is considered to be important in controlling the infection and the outcome of Chagas' disease. Here we show that the potent tumour necrosis factor-alpha-, interleukin-12- and nitric oxide-inducing activities of T.cruzi trypomastigote mucins were recovered quantitatively in a highly purified and characterized glycosylphosphatidylinositol (GPI) anchor fraction of this material. the bioactive trypomastigote GPI fraction was compared with a relatively inactive GPI fraction prepared from T.cruzi epimastigote mucins, the trypomastigote GPI structures were found to contain additional galactose residues and unsaturated, instead of saturated, fatty acids in the sn-2 position of the alkyl-acylglycerolipid component. the latter feature is essential for the extreme potency of the trypomastigote GPI fraction, which is at least as active as bacterial endotoxin and Mycoplasma lipopeptide and, therefore, one of the most potent microbial proinflammatory agents known.
Palavra-chave Chagas' disease
cytokines
glycosylphosphatidylinositol
inflammation
nitric oxide
Idioma Inglês
Data de publicação 2000-04-03
Publicado em Embo Journal. Oxford: Oxford Univ Press, v. 19, n. 7, p. 1476-1485, 2000.
ISSN 0261-4189 (Sherpa/Romeo, fator de impacto)
Publicador Oxford Univ Press
Extensão 1476-1485
Fonte http://dx.doi.org/10.1093/emboj/19.7.1476
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000086388700008
Endereço permanente http://repositorio.unifesp.br/handle/11600/26290

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