Study of acute chagasic mice under immunosuppressive therapy by cyclosporin A: modulation and confocal analysis of inflammatory reaction

Study of acute chagasic mice under immunosuppressive therapy by cyclosporin A: modulation and confocal analysis of inflammatory reaction

Author Calabrese, K. S. Google Scholar
Paradela, ASRC Google Scholar
Valle, T. Z. do Google Scholar
Tedesco, R. C. Google Scholar
Silva, S. Google Scholar
Mortara, R. A. Google Scholar
Costa, SCG da Google Scholar
Institution Ist Oswaldo Cruz
Universidade Federal de São Paulo (UNIFESP)
Abstract The in vivo effects of cyclosporin A (CsA) on Trypanosoma cruzi infection were examined using different schedules of the drug in mice infected with the Y strain. Parasitaemia at day 8 after infection among CsA-treated animals was usually higher than control infected non-treated mice. On the other hand, mortality analysis showed that animals CsA-treated either with 200 mg/kg 2 days before infection or with therapeutic doses (10 mg/kg every other day) showed almost the same mean time of death (35.8 and 38.2 days, respectively). in these groups mice died 50% less than control infected non-treated ones. the mean time of death in the animals treated with 200 mg/kg 5 days after infection and in infected non-treated control mice were respectively 29.0 and 22.6 days. the kinetics analysis of the leukocyte population of animals treated with a single dose of 200 mg/kg of CsA before or after infection did not show the alternate pattern of leukopenia/leukocytosis observed in control groups of infected mice but differential cell counts indicated a modulatory action upon circulating leukocytes of therapeutic doses of CsA. the animals treated with any of the CsA schedules showed a moderate to intense diffuse inflammatory reaction exhibiting mainly mononuclear cells in the heart. Immunofluorescence analysis by confocal microscopy revealed that macrophages are a major component of the inflammatory infiltrate in all groups of CsA-treated mice and also in the control group. (C) 2000 Elsevier Science B.V. All rights reserved.
Keywords immunosuppression
cyclosporin A
acute Chagas' disease
Trypanosoma cruzi
inflammatory reaction
murine model
confocal microscopy
Language English
Date 2000-04-01
Published in Immunopharmacology. Amsterdam: Elsevier B.V., v. 47, n. 1, p. 1-11, 2000.
ISSN 0162-3109 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 1-11
Origin http://dx.doi.org/10.1016/S0162-3109(99)00179-4
Access rights Closed access
Type Article
Web of Science ID WOS:000086010200001
URI http://repositorio.unifesp.br/handle/11600/26280

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