A novel heparan sulphate with high degree of N-sulphation and high heparin cofactor-II activity from the brine shrimp Artemia franciscana

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dc.contributor.author Chavante, S. F.
dc.contributor.author Santos, E. A.
dc.contributor.author Oliveira, F. W.
dc.contributor.author Guerrini, M.
dc.contributor.author Torri, G.
dc.contributor.author Casu, B.
dc.contributor.author Dietrich, C. P.
dc.contributor.author Nader, H. B.
dc.date.accessioned 2016-01-24T12:31:02Z
dc.date.available 2016-01-24T12:31:02Z
dc.date.issued 2000-03-16
dc.identifier http://dx.doi.org/10.1016/S0141-8130(99)00114-2
dc.identifier.citation International Journal of Biological Macromolecules. Amsterdam: Elsevier B.V., v. 27, n. 1, p. 49-57, 2000.
dc.identifier.issn 0141-8130
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/26273
dc.description.abstract With the aid of heparinase and heparitinases from Flavobacterium heparinum and C-13 and H-I NMR spectroscopy it was shown that the heparan sulphate isolated from the brine shrimp Artemia franciscana exhibits structural features intermediate between those of mammalian heparins and heparan sulphates. These include an unusually high degree of N-sulphation with corresponding very low degree of N-acetylation), a relatively high content of iduronic acid residues (both unsulphated and 2-O-sulphated) and a relatively low degree of 6-O-sulphation of the glucosamine residues. the major sequences (glucuronic acid-->N-sulphated glucosamine and glucuronic acid--> N,6-disulphated glucosamine) are most probably arranged in blocks. Although exhibiting negligible anticlotting activity in the APTT and anti-factor Xa assays the A. franciscana heparan sulphate has a high heparin cofactor-II activity (about 1/3 that of heparin). (C) 2000 Elsevier Science B.V. All rights reserved. en
dc.format.extent 49-57
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof International Journal of Biological Macromolecules
dc.rights Acesso restrito
dc.subject brine shrimp heparan sulphate en
dc.subject structure en
dc.subject heparan sulphate en
dc.subject Artemia franciscana en
dc.subject crustacean heparan sulphate en
dc.subject anticlotting activities en
dc.subject heparin cofactor-II activity en
dc.title A novel heparan sulphate with high degree of N-sulphation and high heparin cofactor-II activity from the brine shrimp Artemia franciscana en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Univ Fed Rio Grande Norte
dc.contributor.institution Ist Chim & Biochim G Ronzoni
dc.description.affiliation UNIFESP, Escola Paulista Med, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.affiliation Univ Fed Rio Grande Norte, Dept Bioquim, BR-59072970 Natal, RN, Brazil
dc.description.affiliation Ist Chim & Biochim G Ronzoni, Milan, Italy
dc.description.affiliationUnifesp UNIFESP, Escola Paulista Med, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.identifier.doi 10.1016/S0141-8130(99)00114-2
dc.description.source Web of Science
dc.identifier.wos WOS:000085898300007



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