A novel heparan sulphate with high degree of N-sulphation and high heparin cofactor-II activity from the brine shrimp Artemia franciscana

A novel heparan sulphate with high degree of N-sulphation and high heparin cofactor-II activity from the brine shrimp Artemia franciscana

Author Chavante, S. F. Google Scholar
Santos, E. A. Google Scholar
Oliveira, F. W. Google Scholar
Guerrini, M. Google Scholar
Torri, G. Google Scholar
Casu, B. Google Scholar
Dietrich, C. P. Google Scholar
Nader, H. B. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Fed Rio Grande Norte
Ist Chim & Biochim G Ronzoni
Abstract With the aid of heparinase and heparitinases from Flavobacterium heparinum and C-13 and H-I NMR spectroscopy it was shown that the heparan sulphate isolated from the brine shrimp Artemia franciscana exhibits structural features intermediate between those of mammalian heparins and heparan sulphates. These include an unusually high degree of N-sulphation with corresponding very low degree of N-acetylation), a relatively high content of iduronic acid residues (both unsulphated and 2-O-sulphated) and a relatively low degree of 6-O-sulphation of the glucosamine residues. the major sequences (glucuronic acid-->N-sulphated glucosamine and glucuronic acid--> N,6-disulphated glucosamine) are most probably arranged in blocks. Although exhibiting negligible anticlotting activity in the APTT and anti-factor Xa assays the A. franciscana heparan sulphate has a high heparin cofactor-II activity (about 1/3 that of heparin). (C) 2000 Elsevier Science B.V. All rights reserved.
Keywords brine shrimp heparan sulphate
structure
heparan sulphate
Artemia franciscana
crustacean heparan sulphate
anticlotting activities
heparin cofactor-II activity
Language English
Date 2000-03-16
Published in International Journal of Biological Macromolecules. Amsterdam: Elsevier B.V., v. 27, n. 1, p. 49-57, 2000.
ISSN 0141-8130 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 49-57
Origin http://dx.doi.org/10.1016/S0141-8130(99)00114-2
Access rights Closed access
Type Article
Web of Science ID WOS:000085898300007
URI http://repositorio.unifesp.br/handle/11600/26273

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