A novel heparan sulphate with high degree of N-sulphation and high heparin cofactor-II activity from the brine shrimp Artemia franciscana

A novel heparan sulphate with high degree of N-sulphation and high heparin cofactor-II activity from the brine shrimp Artemia franciscana

Autor Chavante, S. F. Google Scholar
Santos, E. A. Google Scholar
Oliveira, F. W. Google Scholar
Guerrini, M. Google Scholar
Torri, G. Google Scholar
Casu, B. Google Scholar
Dietrich, C. P. Google Scholar
Nader, H. B. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Univ Fed Rio Grande Norte
Ist Chim & Biochim G Ronzoni
Resumo With the aid of heparinase and heparitinases from Flavobacterium heparinum and C-13 and H-I NMR spectroscopy it was shown that the heparan sulphate isolated from the brine shrimp Artemia franciscana exhibits structural features intermediate between those of mammalian heparins and heparan sulphates. These include an unusually high degree of N-sulphation with corresponding very low degree of N-acetylation), a relatively high content of iduronic acid residues (both unsulphated and 2-O-sulphated) and a relatively low degree of 6-O-sulphation of the glucosamine residues. the major sequences (glucuronic acid-->N-sulphated glucosamine and glucuronic acid--> N,6-disulphated glucosamine) are most probably arranged in blocks. Although exhibiting negligible anticlotting activity in the APTT and anti-factor Xa assays the A. franciscana heparan sulphate has a high heparin cofactor-II activity (about 1/3 that of heparin). (C) 2000 Elsevier Science B.V. All rights reserved.
Palavra-chave brine shrimp heparan sulphate
structure
heparan sulphate
Artemia franciscana
crustacean heparan sulphate
anticlotting activities
heparin cofactor-II activity
Idioma Inglês
Data de publicação 2000-03-16
Publicado em International Journal of Biological Macromolecules. Amsterdam: Elsevier B.V., v. 27, n. 1, p. 49-57, 2000.
ISSN 0141-8130 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 49-57
Fonte http://dx.doi.org/10.1016/S0141-8130(99)00114-2
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000085898300007
Endereço permanente http://repositorio.unifesp.br/handle/11600/26273

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