Angiotensin converting-like enzymes from urine of untreated renovascular hypertensive and normal patients: purification and characterization

Angiotensin converting-like enzymes from urine of untreated renovascular hypertensive and normal patients: purification and characterization

Autor Costa, R. H. Google Scholar
Casarini, D. E. Google Scholar
Plavnik, F. L. Google Scholar
Marson, O. Google Scholar
Alves, K. B. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Angiotensin converting-like enzymes (ACE) were isolated from urine of normal (P0N, P1N and P2N) and untreated renovascular hypertensive (P-0, P-1 and P-2) patients, the urine were submitted to ion exchange chromatography. Enzymes P-0 and P0N were eluted with the equilibrium buffer (0.02 M Tris-HCl, pH 7.0), while P-1, P1N, P-2 and P2N with ionic strength linear gradient of 0.02-0.5 M Tris-HCl, pH 7.0 in 0.7 mS and P-2 and P2N in 1.2 mS conductance. the active fractions were submitted to gel filtration in Sephadex G-150, equilibrated and performed with 0.05 M Tris-HCl/0.15 M NaCl buffer, pH 8.0. All enzymes were homogeneous when analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (molecular mass: P-0, P-1 and P2N about 60 kDa; P-1, 95 kDa and P21N 170 kDa). the enzymes were recognized by Y1 polyclonal antibody raised against human renal ACE, the K-M values were in millimolar order for hippuryl-L-His-Leu (HHL) while for benzyloxycarbonyl-Phe-L-His-Leu (ZFHL) they were in 10(-4) M order. the enzymes were able to hydrolyze angiotensin I (AI) (P-0 and P0N about 25%, P-1 and P1N about 70%, P-2 100% and P2N 66%) and bradykinin (BK) (P0N 22%, P1N 81%, P2N 62%, P-0 and P-1 50% and P2 35%), and their activities were inhibited by captopril. (C) 2000 Elsevier Science B.V. All rights reserved.
Palavra-chave angiotensin I-converting enzyme
kininase II
renovascular hypertension
urinary enzymes
Idioma Inglês
Data de publicação 2000-03-01
Publicado em Immunopharmacology. Amsterdam: Elsevier B.V., v. 46, n. 3, p. 237-246, 2000.
ISSN 0162-3109 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 237-246
Fonte http://dx.doi.org/10.1016/S0162-3109(99)00182-4
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000085309100005
Endereço permanente http://repositorio.unifesp.br/handle/11600/26258

Exibir registro completo




Arquivo

Arquivo Tamanho Formato Visualização

Não existem arquivos associados a este item.

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta