Effect of estrogen on intracellular signaling pathways linked to activation of M-2- and M-3-muscarinic acetylcholine receptors in the rat myometrium

Effect of estrogen on intracellular signaling pathways linked to activation of M-2- and M-3-muscarinic acetylcholine receptors in the rat myometrium

Autor Abdalla, FMF Google Scholar
Abreu, L. C. Google Scholar
Porto, C. S. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Inst Butantan
Resumo The estrogen treatment of adult female rats induces an increase in myometrium sensitivity to cholinergic agonists and in this tissue the presence of M-2- and M-3-muscarinic acetylcholine (mACh) receptor was shown. We now report the effect of estrogen on intracellular signaling pathways linked to activation of M-2- and M-3-mACh receptor subtypes. the intracellular cyclic AMP accumulation and [H-3]-inositol phosphates content were measured in myometrium strips from rats in estrus (control) and estradiol-treated rats (12.5 mu g/100 g body weight, sc, 24 h before experiments) (the plasma estradiol level was 30.9 +/- 3.5 pg/ml and 119.3 +/- 14.1 pg/ml from control and estrogen-treated rats, respectively). Estrogen treatment increased 2.5-fold the intracellular cyclic AMP accumulation induced by 10 mu M forskolin. the effects of muscarinic agonist and antagonists on cyclic AMP accumulation were tested. Carbachol reduced the forskolin-induced intracellular cyclic AMP content, 3.0 and 10.5-fold, in myometrium from control and estradiol-treated rats, respectively. This inhibitory effect failed to occur when carbachol was incubated in the presence of methoctramine. Carbachol also induced increase on total [H-3]-inositol phosphates accumulation in myometrium from estradiol-treated rats when compared with control rats. This effect was reversed by pfHHSiD. These studies suggest the modulation by estrogen of intracellular signaling pathways linked to activation of M-2- and M-3-mACh receptors in the rat myometrium. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
Palavra-chave estrogen
muscarinic acetylcholine receptor subtypes
cyclic AMP
inositol phosphate
myometrium
Idioma Inglês
Data de publicação 2000-02-25
Publicado em Molecular and Cellular Endocrinology. Clare: Elsevier Sci Ireland Ltd, v. 160, n. 1-2, p. 17-24, 2000.
ISSN 0303-7207 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 17-24
Fonte http://dx.doi.org/10.1016/S0303-7207(99)00259-2
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000086131300003
Endereço permanente http://repositorio.unifesp.br/handle/11600/26252

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