Purification of porcine plasma factor VIII using chromatographic methods

Purification of porcine plasma factor VIII using chromatographic methods

Autor Tanaka, A. M. Google Scholar
Tanaka, A. S. Google Scholar
Lopes, APY Google Scholar
Takagi, M. Google Scholar
Cabrera-Crespo, J. Google Scholar
Raw, I Google Scholar
Instituição Inst Butantan
Universidade Federal de São Paulo (UNIFESP)
Resumo Factor VIII was purified from porcine plasma using adsorption on aluminium hydroxide with CM-cellulose as a filtration aid, cold ethanol precipitation, and two anion-exchange (Q-Sepharose fast flow) chromatographies. the final product was purified 264-fold and had a specific activity of 10 U mg(-1). the method is suitable to produce purified porcine FVIII by an easy process where all steps can be scaled up. the final product is free of von Willebrand factor that is responsible for the main side effects in patients. Finally, this method can be used to obtain purified porcine plasma FVIII for use in haemophilic patients with inhibitors.
Palavra-chave blood coagulation
chromatography
haemophilia
porcine Factor VIII
protein purification
Idioma Inglês
Data de publicação 2000-02-01
Publicado em Biotechnology Letters. Dordrecht: Kluwer Academic Publ, v. 22, n. 4, p. 257-260, 2000.
ISSN 0141-5492 (Sherpa/Romeo, fator de impacto)
Publicador Kluwer Academic Publ
Extensão 257-260
Fonte http://dx.doi.org/10.1023/A:1005682418076
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000085422700002
Endereço permanente http://repositorio.unifesp.br/handle/11600/26246

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